DETECTION OF GUANINE-C8-2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-B]PYRIDINE ADDUCT AS A SINGLE SPOT ON THIN-LAYER CHROMATOGRAPHY BY MODIFICATION OF THE P-32 POSTLABELING METHOD

被引：53

作者：

FUKUTOME, K

OCHIAI, M

WAKABAYASHI, K

WATANABE, S

SUGIMURA, T

NAGAO, M

机构：

[1] NATL CANC CTR,RES INST,DIV CARCINOGENESIS,CHUO KU,TOKYO 104,JAPAN

[2] SHIGA UNIV MED SCI,DEPT PREVENT MED,OTSU,SHIGA 52021,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1994年 / 85卷 / 02期

关键词：

PHIP-DNA ADDUCT; P-32 POSTLABELING METHOD; MAJOR ADDUCT; 5'-PDG-C8-PHIP;

D O I：

10.1111/j.1349-7006.1994.tb02068.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

N-(Deoxyguanosin-8-yl)-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (dG-C8-PhIP) has been shown to be a major adduct in DNA of rats given [H-3]PhIP. However, when DNA from organs of rats fed PhIP was analyzed by the P-32-postlabeling method under standard and adduct-intensification conditions, four adduct spots were observed, and 3',5'-pdGp-C8-PhIP was detected as a minor, not a major, adduct spot. Since the three other major adduct spots were suspected to be those of adducted di- or oligo-nucleotides, the P-32-labeled samples were further treated with nuclease P1 and phosphodiesterase I and found to yield only a single adduct spot. The material in this adduct spot was confirmed to be 5'-pdG-CS-PhIP. Thus, using this newly modified P-32-postlabeling method, dG-C8-PhIP was detected as a major adduct in DNA of rats given PhIP.

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页码：113 / 117

页数：5

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