Solid-phase microextraction-liquid chromatography (SPME-LC) determination of fluoxetine and norfluoxetine in plasma using a heated liquid flow through interface

被引：48

作者：

Fernandes, Christian ^{[1
]}

Santos-Neto, Alvaro Jose ^{[1
]}

Rodrigues, Jose Carlos ^{[1
]}

Alves, Claudete ^{[1
]}

Lancas, Fernando Mauro ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Chem, BR-13566590 Sao Carlos, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 847卷 / 02期

关键词：

SPME; SPME-LC; interface; fluoxetine; plasma;

D O I：

10.1016/j.jchromb.2006.10.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive procedure using solid-phase microextraction coupled with high performance liquid chromatography (HPLC) to analyze fluoxetine (FLU) and its metabolite norfluoxetine (nor-FLU) in plasma samples was developed and validated. SPME conditions were optimized employing a factorial design. The sampling step was performed using a PDMS-DV13 fiber and desorption was carried out in a novel homemade heated interface. Fluoxetine and norfluoxetine were analyzed by HPLC, using a C18 Phase Sep column (150 mm, x 4.6 mm, 3 mu m) packed "in house", and acetonitrile: acetate buffer 25 mmol 1(-1) with triethylamine 25 mmol l(-1) pH 4.6 (70:30) as the mobile phase. The developed method has shown precision, linearity, specificity, and limit of quantification (LOQ) adequate to assay fluoxetine and norfluoxetine in plasma. Furthermore, the results obtained using the homemade interface has shown an improvement in the desorption process when compared with the results obtained using the off-line mode. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：217 / 223

页数：7