Convenient synthesis of NCS-chromophore metcabolite isosteres: Binding agents for bulged DNA microenvironments

被引：28

作者：

Lin, YQ ^{[1
]}

Jones, GB ^{[1
]}

Hwang, GS ^{[1
]}

Kappen, L ^{[1
]}

Goldberg, IH ^{[1
]}

机构：

[1] Northeastern Univ, Dept Chem & Biol Chem, Bioorgan & Med Chem Labs, Boston, MA 02115 USA

来源：

ORGANIC LETTERS | 2005年 / 7卷 / 01期

关键词：

D O I：

10.1021/ol0400591

中图分类号：

O62 [有机化学];

学科分类号：

070303 [有机化学]; 081704 [应用化学];

摘要：

A designed molecule with capacity to bind DNA bulges (20) has been prepared from readily available starting materials. The spirocyclic template was modeled on a metabolite of neocarzinostatin chromophore (NCSi-gb) and is equipped with functionality to enable convenient bioassay. Preliminary studies confirm binding at specific bulged sequences and induction of polymerase-mediated slippage events. The target compound offers a convenient means to study affinity for unique bulged motifs and for use as a molecular biology reagent.

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页码：71 / 74

页数：4

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