Advances in fluorescent in situ hybridisation

被引:18
作者
Ekong, R
Wolfer, J
机构
[1] MRC, Human Biochem Genet Unit, London NW1 2HE, England
[2] UCL, Dept Biol, Galton Lab, London NW1 2HE, England
关键词
D O I
10.1016/S0958-1669(98)80079-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Recent advances in fluorescent in situ hybridisation included the generation of allele-specific probes, bar-coded chromosomes, and the visualisation of chromosome territories and genes within the nucleus. One major advance has been our ability to visualise and make precise and reproducible measurements from stretched DNA molecules prepared directly from human cells. (C) Current Biology Ltd ISSN 0958-1669.
引用
收藏
页码:19 / 24
页数:6
相关论文
共 49 条
  • [1] Telomeres cluster de novo before the initiation of synapsis: A three-dimensional spatial analysis of telomere positions before and during meiotic prophase
    Bass, HW
    Marshall, WF
    Sedat, JW
    Agard, DA
    Cande, WZ
    [J]. JOURNAL OF CELL BIOLOGY, 1997, 137 (01) : 5 - 18
  • [2] ALIGNMENT AND SENSITIVE DETECTION OF DNA BY A MOVING INTERFACE
    BENSIMON, A
    SIMON, A
    CHIFFAUDEL, A
    CROQUETTE, V
    HESLOT, F
    BENSIMON, D
    [J]. SCIENCE, 1994, 265 (5181) : 2096 - 2098
  • [3] Bicolor fluorescence in situ hybridization on nuclei from formalin-fixed, paraffin-embedded testicular germ cell tumors: Comparison with standard metaphase analysis
    Blough, RI
    Smolarek, TA
    Ulbright, TM
    Heerema, NA
    [J]. CANCER GENETICS AND CYTOGENETICS, 1997, 94 (02) : 79 - 84
  • [4] Removal of repetitive sequences from FISH probes using PCR-assisted affinity chromatography
    Craig, JM
    Kraus, J
    Cremer, T
    [J]. HUMAN GENETICS, 1997, 100 (3-4) : 472 - 476
  • [5] Multicolour FISH detects frequent chromosomal mosaicism and chaotic division in normal preimplantation embryos from fertile patients
    Delhanty, JDA
    Harper, JC
    Ao, A
    Handyside, AH
    Winston, RML
    [J]. HUMAN GENETICS, 1997, 99 (06) : 755 - 760
  • [6] Generation of small insert genomic FISH probes with high signal intensity suitable for deletion mapping
    Dreyling, MH
    Olopade, OI
    Bohlander, SK
    [J]. CYTOGENETICS AND CELL GENETICS, 1997, 76 (3-4): : 202 - 205
  • [7] Three-dimensional reconstruction of painted human interphase chromosomes: Active and inactive X chromosome territories have similar volumes but differ in shape and surface structure
    Eils, R
    Dietzel, S
    Bertin, E
    Schrock, E
    Speicher, MR
    Ried, T
    RobertNicoud, M
    Cremer, C
    Cremer, T
    [J]. JOURNAL OF CELL BIOLOGY, 1996, 135 (06) : 1427 - 1440
  • [8] Assignment of the beta-nerve growth factor (NGFB) to bovine chromosome 3 band q23 by in situ hybridization
    Elduque, C
    Laurent, P
    Hayes, H
    Rodellar, C
    Leveziel, H
    Zaragoza, P
    [J]. CYTOGENETICS AND CELL GENETICS, 1997, 77 (3-4): : 306 - 307
  • [9] 2 SIMPLE PROCEDURES FOR RELEASING CHROMATIN FROM ROUTINELY FIXED CELLS FOR FLUORESCENCE IN-SITU HYBRIDIZATION
    FIDLEROVA, H
    SENGER, G
    KOST, M
    SANSEAU, P
    SHEER, D
    [J]. CYTOGENETICS AND CELL GENETICS, 1994, 65 (03): : 203 - 205
  • [10] Design and validation of DNA probe sets for a comprehensive interphase cytogenetic analysis of acute myeloid leukemia
    Fischer, K
    Scholl, C
    Salat, J
    Frohling, S
    Schlenk, R
    Bentz, M
    Stilgenbauer, S
    Lichter, P
    Dohner, H
    [J]. BLOOD, 1996, 88 (10) : 3962 - 3971