Sense transformation reveals a novel role for class I β-1,3-glucanase in tobacco seed germination

被引:70
作者
Leubner-Metzger, G [1 ]
Meins, F [1 ]
机构
[1] Friedrich Miescher Inst, CH-4058 Basel, Switzerland
关键词
abscisic acid; after-ripening; endosperm-limited seed germination; gene function; beta-1,3-glucanase; sense transformation;
D O I
10.1046/j.1365-313x.2000.00773.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
'Coat-enhanced' seed dormancy of many dicotyledonous species, including tobacco, is released during after-ripening. Rupture of the endosperm, which is the limiting step in tobacco seed germination, is preceded by induction of class I beta-1,3-glucanase (beta GLU I) in the micropylar endosperm where the radicle will penetrate. Treating after-ripened tobacco seeds with abscisic acid (ABA) delays endosperm rupture and inhibits beta GLU I induction. Sense transformation with a chimeric ABA-inducible beta GLU I transgene resulted in over-expression of beta GLU I in seeds and promoted endosperm rupture of mature seeds and of ABA-treated after-ripened seeds. Taken together, these results provide direct evidence that beta GLU I contributes to endosperm rupture. Over-expression of beta GLU I during germination also replaced the effects of after-ripening on endosperm rupture. This suggests that regulation of beta GLU I by ABA signalling pathways might have a key role in after-ripening.
引用
收藏
页码:215 / 221
页数:7
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