Fractionation of apple procyanidins according to their degree of polymerization by normal-phase high-performance liquid chromatography

被引：65

作者：

Yanagida, A

Kanda, T

Takahashi, T

Kamimura, A

Hamazono, T

Honda, S

机构：

[1] Nikka Whisky Distilling Co Ltd, Inst Prod Res & Dev, Kashiwa, Chiba 2770033, Japan

[2] Kyowa Hakko Kogyo Co Ltd, Tsukuba Res Labs, Tsukuba, Ibaraki 3050841, Japan

[3] Kyowa Hakko Kogyo Co Ltd, Biochem Co, Dept Dev, Tokyo 1008185, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 890卷 / 02期

关键词：

apples; tannins; procyanidins;

D O I：

10.1016/S0021-9673(00)00614-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method was developed for the fractionation of procyanidin oligomers according to their degree of polymerization. Monomeric flavan-3-ols and low molecular mass procyanidins were selectively extracted from the lyophilized powder of apple condensed tannins (ACTs) by methyl acetate extraction. Sequentially, the separation of each oligomer from dimer to pentamer in this extract was carried out by normal-phase high-performance liquid chromatography using a silica-beads packed column. The best separation was achieved with a mobile phase system containing hexane; (1) hexane-methanol-ethyl acetate, (2) hexane-acetone, These sequential treatments can be easily adapted to large-scale fractionation. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：251 / 259

页数：9

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