Antibody validation

被引：440

作者：

Bordeaux, Jennifer ^{[1
]}

Welsh, Allison W. ^{[1
]}

Agarwal, Seema ^{[1
]}

Killiam, Elizabeth ^{[1
]}

Baquero, Maria T. ^{[1
]}

Hanna, Jason A. ^{[1
]}

Anagnostou, Valsamo K. ^{[1
]}

Rimm, David L. ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06520 USA

来源：

BIOTECHNIQUES | 2010年 / 48卷 / 03期

基金：

美国国家卫生研究院;

关键词：

antibody; validation; immunohistochemistry; immunofluorescence; RABBIT MONOCLONAL-ANTIBODY; BREAST-CANCER; PHOSPHORYLATION STATE; COMMERCIAL ANTIBODIES; ESTROGEN-RECEPTOR; TISSUE-SECTIONS; IMMUNOHISTOCHEMISTRY; STANDARDIZATION; PROTEIN; RECOMMENDATIONS;

D O I：

10.2144/000113382

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies are among the most frequently used tools in basic science research and in clinical assays, but there are no universally accepted guidelines or standardized methods for determining the validity of these reagents. Furthermore, for commercially available antibodies, it is clear that what is on the label does not necessarily correspond to what is in the tube. To validate an antibody, it must be shown to be specific, selective, and reproducible in the context for which it is to be used. In this review, we highlight the common pitfalls when working with antibodies, common practices for validating antibodies, and levels of commercial antibody validation for seven vendors. Finally, we share our algorithm for antibody validation for immunohistochemistry and quantitative immunofluorescence.

引用

页码：197 / 209

页数：8

共 41 条

[1] Antibodypedia, a Portal for Sharing Antibody and Antigen Validation Data [J].

Bjorling, Erik ;

Uhlen, Mathias .

MOLECULAR & CELLULAR PROTEOMICS, 2008, 7 (10) :2028-2037

[2] A Comparison of Estrogen Receptor SP1 and 1D5 Monoclonal Antibodies in Routine Clinical Use Reveals Similar Staining Results [J].

Brock, Jane E. ;

Hornick, Jason L. ;

Richardson, Andrea L. ;

Dillon, Deborah A. ;

Lester, Susan C. .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2009, 132 (03) :396-401

[3] A Decade of Tissue Microarrays: Progress in the Discovery and Validation of Cancer Biomarkers [J].

Camp, Robert L. ;

Neumeister, Veronique ;

Rimm, David L. .

JOURNAL OF CLINICAL ONCOLOGY, 2008, 26 (34) :5630-5637

[4] Immunohistochemical detection using the new rabbit monoclonal antibody SP1 of estrogen receptor in breast cancer is superior to mouse monoclonal antibody 1D5 in predicting survival [J].

Cheang, Maggie C. U. ;

Treaba, Diana O. ;

Speers, Caroline H. ;

Olivotto, Ivo A. ;

Bajdik, Chris D. ;

Chia, Stephen K. ;

Goldstein, Lynn C. ;

Gelmon, Karen A. ;

Huntsman, David ;

Gilks, C. Blake ;

Nielsen, Torsten O. ;

Gown, Allen M. .

JOURNAL OF CLINICAL ONCOLOGY, 2006, 24 (36) :5637-5644

[5] Commercial Antibodies: The Good, Bad, and Really Ugly [J].

Couchman, John R. .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2009, 57 (01) :7-8

[6] Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE) [J].

Deutsch, Eric W. ;

Ball, Catherine A. ;

Berman, Jules J. ;

Bova, G. Steven ;

Brazma, Alvis ;

Bumgarner, Roger E. ;

Campbell, David ;

Causton, Helen C. ;

Christiansen, Jeffrey H. ;

Daian, Fabrice ;

Dauga, Delphine ;

Davidson, Duncan R. ;

Gimenez, Gregory ;

Goo, Young Ah ;

Grimmond, Sean ;

Henrich, Thorsten ;

Herrmann, Bernhard G. ;

Johnson, Michael H. ;

Korb, Martin ;

Mills, Jason C. ;

Oudes, Asa J. ;

Parkinson, Helen E. ;

Pascal, Laura E. ;

Pollet, N. Icolas ;

Quackenbush, John ;

Ramialison, Mirana ;

Ringwald, Martin ;

Salgado, David ;

Sansone, Susanna-Assunta ;

Sherlock, Gavin ;

Stoeckert, Christian J., Jr. ;

Swedlow, Jason ;

Taylor, Ronald C. ;

Walashek, Laura ;

Warford, Anthony ;

Wilkinson, David G. ;

Zhou, Yi ;

Zon, Leonard I. ;

Liu, Alvin Y. ;

True, Lawrence D. .

NATURE BIOTECHNOLOGY, 2008, 26 (03) :305-312

[7] Standardization of HER2 testing:: results of an international proficiency-testing ring study [J].

Dowsett, Mitch ;

Hanna, Wedad M. ;

Kockx, Mark ;

Penault-Llorca, Frederique ;

Rueschoff, Josef ;

Gutjahr, Thorsten ;

Habben, Kai ;

van de Vijver, Marc J. .

MODERN PATHOLOGY, 2007, 20 (05) :584-591

[8] Insights into ErbB signaling from the structure of the ErbB2-pertuzumab complex [J].

Franklin, MC ;

Carey, KD ;

Vajdos, FF ;

Leahy, DJ ;

de Vos, AM ;

Sliwkowski, MX .

CANCER CELL, 2004, 5 (04) :317-328

[9] Recommendations for improved standardization of immunohistochemistry [J].

Goldstein, Neal S. ;

Hewitt, Stephen M. ;

Taylor, Clive R. ;

Yaziji, Hadi ;

Hicks, David G. .

APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2007, 15 (02) :124-133

[10] Specific detection of CB1 receptors;: cannabinoid CB1 receptor antibodies are not all created equal! [J].

Grimsey, Natasha L. ;

Goodfellow, Catherine E. ;

Scotter, Emma L. ;

Dowie, Megan J. ;

Glass, Michelle ;

Graham, E. Scott .

JOURNAL OF NEUROSCIENCE METHODS, 2008, 171 (01) :78-86

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