Interferon-beta and interferon-gamma synergistically inhibit the replication of severe acute respiratory syndrome-associated coronavirus (SARS-CoV)

被引:143
作者
Sainz, B
Mossel, EC
Peters, CJ
Garry, RF
机构
[1] Univ Texas, Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA
[2] Tulane Univ, Hlth Sci Ctr, Dept Microbiol & Immunol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA
[3] Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA
关键词
coronavirus; interferon-gamma; interferon-beta;
D O I
10.1016/j.virol.2004.08.011
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Recent studies have shown that interferon-gamma (IFN-gamma) synergizes with IFN-alpha/beta to inhibit the replication of both RNA and DNA viruses. We investigated the effects of IFNs on the replication of two strains of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). While treatment of Vero E6 cells with 100 U/ml of either IFN-beta or IFN-gamma marginally reduced viral replication, treatment with both IFN-beta and IFN-gamma inhibited SARS-CoV plaque formation by 30-fold and replication by 3000-fold at 24 h and by > 1 X 10(5)-fold at 48 and 72 h post-infection. These studies suggest that combination IFN treatment warrants further investigation as a treatment for SARS. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:11 / 17
页数:7
相关论文
共 20 条
[11]   A novel coronavirus associated with severe acute respiratory syndrome [J].
Ksiazek, TG ;
Erdman, D ;
Goldsmith, CS ;
Zaki, SR ;
Peret, T ;
Emery, S ;
Tong, SX ;
Urbani, C ;
Comer, JA ;
Lim, W ;
Rollin, PE ;
Dowell, SF ;
Ling, AE ;
Humphrey, CD ;
Shieh, WJ ;
Guarner, J ;
Paddock, CD ;
Rota, P ;
Fields, B ;
DeRisi, J ;
Yang, JY ;
Cox, N ;
Hughes, JM ;
LeDuc, JW ;
Bellini, WJ ;
Anderson, LJ .
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1953-1966
[12]  
LARKIN J, 2003, J INTERFERON CYTOKIN, V23, P547
[13]   Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus [J].
Li, WH ;
Moore, MJ ;
Vasilieva, N ;
Sui, JH ;
Wong, SK ;
Berne, MA ;
Somasundaran, M ;
Sullivan, JL ;
Luzuriaga, K ;
Greenough, TC ;
Choe, H ;
Farzan, M .
NATURE, 2003, 426 (6965) :450-454
[14]   Interferon alfacon-1 plus corticosteroids in severe acute respiratory syndrome - A preliminary study [J].
Loutfy, MR ;
Blatt, LM ;
Siminovitch, KA ;
Ward, S ;
Wolff, B ;
Lho, H ;
Pham, DH ;
Deif, H ;
LaMere, EA ;
Chang, M ;
Kain, KC ;
Farcas, GA ;
Ferguson, P ;
Latchford, M ;
Levy, G ;
Dennis, JW ;
Lai, EKY ;
Fish, EN .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2003, 290 (24) :3222-3228
[15]   Coronavirus as a possible cause of severe acute respiratory syndrome [J].
Peiris, JSM ;
Lai, ST ;
Poon, LLM ;
Guan, Y ;
Yam, LYC ;
Lim, W ;
Nicholls, J ;
Yee, WKS ;
Yan, WW ;
Cheung, MT ;
Cheng, VCC ;
Chan, KH ;
Tsang, DNC ;
Yung, RWH ;
Ng, TK ;
Yuen, KY .
LANCET, 2003, 361 (9366) :1319-1325
[16]  
Pfeffer LM, 1998, CANCER RES, V58, P2489
[17]   Alpha/beta interferon and gamma interferon synergize to inhibit the replication of herpes simplex virus type 1 [J].
Sainz, B ;
Halford, WP .
JOURNAL OF VIROLOGY, 2002, 76 (22) :11541-11550
[18]  
Spies JJ, 2000, BOTHALIA, V30, P211
[19]   Severe acute respiratory syndrome related coronavirus is inhibited by interferon-α [J].
Ströher, U ;
DiCaro, A ;
Li, Y ;
Strong, JE ;
Aoki, F ;
Plummer, F ;
Jones, SM ;
Feldmann, H .
JOURNAL OF INFECTIOUS DISEASES, 2004, 189 (07) :1164-1167
[20]   SARS-related virus predating SARS outbreak, Hong Kong [J].
Zheng, BJ ;
Guan, Y ;
Wong, KH ;
Zhou, J ;
Wong, KL ;
Young, BWY ;
Lu, LW ;
Lee, SS .
EMERGING INFECTIOUS DISEASES, 2004, 10 (02) :176-178