Assay for ubiquitin ligase activity: High-throughput screen for inhibitors of HDM2

被引：40

作者：

Davydov, IV

Woods, D

Safiran, YJ

Oberoi, P

Fearnhead, HO

Fang, S

Jensen, JP

Weissman, AM

Kenten, JH

Vousden, KH

机构：

[1] Meso Scale Diagnost LLC, Meso Scale Discovery, Gaithersburg, MD USA

[2] NCI, Regulat Cell Growth Lab, Canc Res Ctr, Frederick, MD 21701 USA

[3] NCI, Lab Prot Dynam & Signaling, Ctr Canc Res, Frederick, MD 21701 USA

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2004年 / 9卷 / 08期

关键词：

HDM2; Mdm2; ubiquitin; E3; p53;

D O I：

10.1177/1087057104267956

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay for the autoubiquitination activity of the E3 ligase HDM2 (Mdm2) was developed and adapted to a high-throughput formal to identify inhibitors of this activity. The assay can also be used to measure the activity of other E3s and may be useful in finding both inhibitors and activators of a wide range of different ubiquitin ligases.

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收藏

页码：695 / 703

页数：9

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