Profile of cytokine mRNA expression in peri-implantation human endometrium

被引:89
作者
Lim, KJH
Odukoya, OA
Ajjan, RA
Li, TC
Weetman, AP
Cooke, ID
机构
[1] Univ Sheffield, Jessop Hosp Women, Dept Obstet & Gynaecol, Sheffield S3 7RE, S Yorkshire, England
[2] Univ Sheffield, No Gen Hosp, Dept Med, Ctr Clin Sci, Sheffield S5 7AU, S Yorkshire, England
关键词
cytokines; endometrium; implantation;
D O I
10.1093/molehr/4.1.77
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The human embryo and subsequent fetus is a semi-allograft and immunologically foreign to the mother. Successful embryonic implantation and maintenance of normal pregnancy depends on intricate interactions between the invading embryo, maternal endometrium and maternal-immune response. Cytokines are pivotal in determining the type of maternal T-helper immune response, which can be divided into T-helper 1 (TH1)-mediated delayed hypersensitivity and T-helper 2 (TH2)-mediated humoral immunity. We examined the pattern of TH1 [interleukin (IL)-2, IL-12 and interferon-gamma] and TH2 (IL-4 and IL-6) cytokine expression in the peri-implantation endometrium of 10 fertile, multiparous women. Endometrial samples were timed to the mid-cycle luteinizing hormone (LH) surge, with collection on days LH+7 to LH+9. Analysis for cytokine mRNA was by mRNA extraction and reverse transcription-polymerase chain reaction. The results show a lack of TH1 and predominant TH2 cytokine expression. We postulate that endometrial cytokines may contribute to the local modulation of maternal adaptive immune response in peri-implantation endometrium, to facilitate successful embryonic implantation and maintenance of pregnancy.
引用
收藏
页码:77 / 81
页数:5
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