Ab Initio Structure Determination from Electron Microscopic Images of Single Molecules Coexisting in Different Functional States

被引：37

作者：

Elmlund, Dominika ^{[2
,4
,5
]}

Davis, Ralph ^{[1
]}

Elmlund, Hans ^{[1
,3
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

[2] Karolinska Inst, Dept Lab Med, SE-14186 Huddinge, Sweden

[3] Gothenburg Univ, Dept Med Biochem & Cell Biol, SE-40530 Gothenburg, Sweden

[4] Karolinska Inst, Dept Biosci & Nutr, SE-14187 Huddinge, Sweden

[5] Royal Inst Technol, Sch Technol & Hlth, SE-14187 Huddinge, Sweden

来源：

STRUCTURE | 2010年 / 18卷 / 07期

基金：

瑞典研究理事会;

关键词：

RNA-POLYMERASE-II; CRYO-EM REVEALS; CONFORMATIONAL FLEXIBILITY; ORIENTATION REFINEMENT; COMMON-LINES; RECONSTRUCTION; CLASSIFICATION; TRANSCRIPTION; PROJECTIONS; RESOLUTION;

D O I：

10.1016/j.str.2010.06.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed methods for ab initio three-dimensional (3D) structure determination from projection images of randomly oriented single molecules coexisting in multiple functional states, to aid the study of complex samples of macromolecules and nanoparticles by electron microscopy (EM). New algorithms for the determination of relative 3D orientations and conformational state assignment of single-molecule projection images are combined with well-established techniques for alignment and statistical image analysis. We describe how the methodology arrives at homogeneous groups of images aligned in 3D and discuss application to experimental EM data sets of the Escherichia coli ribosome and yeast RNA polymerase II.

引用

页码：777 / 786

页数：10

共 56 条

[1] CRYO-ELECTRON MICROSCOPY OF VIRUSES [J].