Genomic organization, chromosomal assignment, and expression analysis of the mouse Suppressor of fused gene (Sufu) coding a Gli protein partner

被引：17

作者：

Simon-Chazottes, D

Paces-Fessy, M

Lamour-Isnard, C

Guénet, JL

Blanchet-Tournier, MF

机构：

[1] Univ Paris 06, Inst Jacques Monod, Lab Genet Dev & Evolut, F-75251 Paris 05, France

[2] Univ Denis Diderot, Inst Jacques Monod, Lab Genet Dev & Evolut, F-75251 Paris, France

[3] Inst Pasteur, Unite Genet Mammiferes, F-75724 Paris 15, France

来源：

MAMMALIAN GENOME | 2000年 / 11卷 / 08期

关键词：

D O I：

10.1007/s003350010144

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Suppressor of fused (Sufu) is a negative regulator of the Hedgehog pathway both in Drosophila and vertebrates. Here, we report the genomic organization of the mouse Sufu gene (mSufu). This gene comprises 11 exons spanning more than 30 kb and encodes a protein with a putative PEST sequence. DNA-consensus sequences recognized by basic helix-loop-helix (bHLH) proteins, referred to as E-box motifs, are found in the 5' flanking region. Analysis by single-strand conformation polymorphism and radiation hybrid positioned the Sufu locus to the distal end of mouse Chr 19 between D19Mit102 and D19Mit9, near the Fgf8 and dactylin genes. Mouse Sufu is expressed in various tissues, particularly in the nervous system, ectoderm, and limbs, throughout the developing embryo. Sufu binds with all three Gli proteins, with different affinities. This report, in conjunction with recent studies, points out the importance of SuFu in mouse embryonic development.

引用

收藏

页码：614 / 621

页数：8

相关论文

共 39 条

[1] Transcriptional activation of hedgehog target genes in Drosophila is mediated directly by the cubitus interruptus protein, a member of the GLI family of zinc finger DNA-binding proteins [J].

Alexandre, C ;

Jacinto, A ;

Ingham, PW .

GENES & DEVELOPMENT, 1996, 10 (16) :2003-2013

[2] Proteolysis that is inhibited by Hedgehog targets Cubitus interruptus protein to the nucleus and converts it to a repressor [J].

AzaBlanc, P ;

RamirezWeber, FA ;

Laget, MP ;

Schwartz, C ;

Kornberg, TB .

CELL, 1997, 89 (07) :1043-1053

[3] TOWARDS HIGH-RESOLUTION MAPS OF THE MOUSE AND HUMAN GENOMES - A FACILITY FOR ORDERING MARKERS TO 0.1 CM RESOLUTION [J].

BREEN, M ;

DEAKIN, L ;

MACDONALD, B ;

MILLER, S ;

SIBSON, R ;

TARTTELIN, E ;

AVNER, P ;

BOURGADE, F ;

GUENET, JL ;

MONTAGUTELLI, X ;

POIRIER, C ;

SIMON, D ;

TAILOR, D ;

BISHOP, M ;

KELLY, M ;

RYSAVY, F ;

RASTAN, S ;

NORRIS, D ;

SHEPHERD, D ;

ABBOTT, C ;

PILZ, A ;

HODGE, S ;

JACKSON, I ;

BOYD, Y ;

BLAIR, H ;

MASLEN, G ;

TODD, JA ;

REED, PW ;

STOYE, J ;

ASHWORTH, A ;

MCCARTHY, L ;

COX, R ;

SCHALKWYK, L ;

LEHRACH, H ;

KLOSE, J ;

GANGADHARAN, U ;

BROWN, S .

HUMAN MOLECULAR GENETICS, 1994, 3 (04) :621-627

[4] Nuclear trafficking of cubitus interruptus in the transcriptional regulation of hedgehog target gene expression [J].

Chen, CH ;

von Kessler, DP ;

Park, WJ ;

Beachy, PA .

CELL, 1999, 98 (03) :305-316

[5] Protein kinase A directly regulates the activity and proteolysis of cubitus interruptus [J].

Chen, Y ;

Gallaher, N ;

Goodman, RH ;

Smolik, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :2349-2354

[6] Activation of the transcription factor Gli1 and the Sonic hedgehog signalling pathway in skin tumours [J].

Dahmane, N ;

Lee, J ;

Robins, P ;

Heller, P ;

Altaba, ARI .

NATURE, 1997, 389 (6653) :876-881

[7]

DAHMANE N, 1997, IN PRESS NATURE, V4, P390

[8] The Suppressor of fused gene, involved in Hedgehog signal transduction in Drosophila, is conserved in mammals [J].

Delattre, M ;

Briand, S ;

Paces-Fessy, M ;

Blanchet-Tournier, MF .

DEVELOPMENT GENES AND EVOLUTION, 1999, 209 (05) :294-300

[9] Mouse Suppressor of fused is a negative regulator of Sonic hedgehog signaling and alters the subcellular distribution of Gli1 [J].

Ding, Q ;

Fukami, SI ;

Meng, X ;

Nishizaki, Y ;

Zhang, X ;

Sasaki, H ;

Dlugosz, A ;

Nakafuku, M ;

Hui, CC .

CURRENT BIOLOGY, 1999, 9 (19) :1119-1122

[10] Sending and receiving the Hedgehog signal: Control by the Drosophila Gli protein Cubitus interruptus [J].

Dominguez, M ;

Brunner, M ;

Hafen, E ;

Basler, K .

SCIENCE, 1996, 272 (5268) :1621-1625

← 1 2 3 4 →