Differential expression of photosynthesis-related genes of reed ecotypes in response to drought and saline habitats

被引：22

作者：

Wang, HL ^{[1
]}

Hao, LM ^{[1
]}

Wen, JQ ^{[1
]}

Zhang, CL ^{[1
]}

Liang, HG ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Biol, Lanzhou 730000, Peoples R China

来源：

PHOTOSYNTHETICA | 1998年 / 35卷 / 01期

关键词：

cab; 16S rDNA; net photosynthetic rate; Phragmites communis; psbA; stomatal conductance;

D O I：

10.1023/A:1006817714739

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Three ecotypes of reed (Phragmites communis Trinius), swamp reed (SR), dune reed (DR), and heavy salt meadow reed (HSMR), growing in desert regions of northwest China were simultaneously investigated in their natural state for gas exchange patterns and the expression of three photosynthesis-related genes, cab (the gene for the light-harvesting chlorophyll a/b binding protein, LHC), psbA (the gene for the reaction centre D1 protein of photosystem 2, PS2), and 16S rDNA (the gene for plastid 16S rRNA). Stomatal conductance (g(s)) and intercellular CO2 concentration (c(i)) were markedly lower in the two terrestrial ecotypes (DR and HSMR) as compared to SR, paralleling a similar observed depression in net photosynthetic rate (P-N) However, DR with the lowest measured g(s) and c(i) still exhibited a higher P-N compared to HSMR. These results suggest that both stomatal and non-stomatal factors account for the comparatively low carbon assimilation in the terrestrial ecotypes. An increase in the expression of photosynthesis-related genes was observed in DR compared to SR, whereas the reverse situation was true in HSMR. The expression of photosynthesis-related genes may contribute to reed plants' photosynthetic capacity per leaf area under natural water deficits, but the levels of photosynthesis-related gene expression are not directly correlated with reed plants' general ability for survival and adaptation under water deficient conditions.

引用

页码：61 / 69

页数：9

共 32 条

[11] INCREASING SALT TOLERANCE OF PLANTS THROUGH CELL-CULTURE REQUIRES GREATER UNDERSTANDING OF TOLERANCE MECHANISMS [J].

DRACUP, M .

AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1991, 18 (01) :1-15

[12] PEPTIDE AND PROTEIN MOLECULAR-WEIGHT DETERMINATION BY ELECTROPHORESIS USING A HIGH-MOLARITY TRIS BUFFER SYSTEM WITHOUT UREA [J].

FLING, SP ;

GREGERSON, DS .

ANALYTICAL BIOCHEMISTRY, 1986, 155 (01) :83-88

[13] Effects of water stress on light-harvesting complex II (LHCII) and expression of a gene encoding LHCII in Zea mays [J].

Hao, LM ;

Wang, HL ;

Wen, JQ ;

Liang, HG .

JOURNAL OF PLANT PHYSIOLOGY, 1996, 149 (1-2) :30-34

[14] DEVELOPMENT AND EMERGENCE OF BUDS IN PHRAGMITES COMMUNIS TRIN [J].

HASLAM, SM .

ANNALS OF BOTANY, 1969, 33 (130) :289-&

[15]

HE JX, 1995, PHYSIOL PLANTARUM, V93, P771, DOI 10.1111/j.1399-3054.1995.tb05130.x

[16] A RAPID BOILING METHOD FOR THE PREPARATION OF BACTERIAL PLASMIDS [J].

HOLMES, DS ;

QUIGLEY, M .

ANALYTICAL BIOCHEMISTRY, 1981, 114 (01) :193-197

[17]

KARPINSKI S, 1994, PHYSIOL PLANTARUM, V90, P358, DOI 10.1111/j.1399-3054.1994.tb00400.x

[18] CHLOROPLAST RESPONSE TO LOW LEAF WATER POTENTIALS .3. DIFFERING INHIBITION OF ELECTRON-TRANSPORT AND PHOTOPHOSPHORYLATION [J].

KECK, RW ;

BOYER, JS .

PLANT PHYSIOLOGY, 1974, 53 (03) :474-479

[19]

LANGE OL, 1981, PHYSL PLANT ECOLOGY, V1, P1

[20] IMPROVED METHOD FOR THE ISOLATION OF RNA FROM PLANT-TISSUES [J].

LOGEMANN, J ;

SCHELL, J ;

WILLMITZER, L .

ANALYTICAL BIOCHEMISTRY, 1987, 163 (01) :16-20

← 1 2 3 4 →