Identification of genes associated with tumorigenesis of retinoblastoma by microarray analysis

被引：98

作者：

Chakraborty, Sanjukta

Khare, Sonal

Dorairaj, Syril Kumar

Prabhakaran, Venkatesh C.

Prakash, D. Ravi

Kumar, Arun ^{[1
]}

机构：

[1] Indian Inst Sci, Dept Mol Reprod Dev & Genet, Bangalore 560012, Karnataka, India

[2] Minto Ophthalm Hosp, Bangalore, Karnataka, India

来源：

GENOMICS | 2007年 / 90卷 / 03期

关键词：

expression profile; retinoblastoma; microarrays; insulin signaling pathway;

D O I：

10.1016/j.ygeno.2007.05.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

There is no report on the gene expression profile of retinoblastoma (Rb). We analyzed the gene expression profile of Rb by the microarray technique. One thousand four genes were upregulated and 481 genes were downregulated. Microarray data were confirmed by semiquantitative RT-PCR for 5 genes in Rb samples: CDC25A, C17orf75, ERBB3, LATS2, and CHFR. Clusters of differentially expressed genes were identified on chromosomes 1, 16, and 17. Based on the expression profile, we hypothesized that the PI3K/AKT/mTOR (insulin signaling) pathway might be dysregulated in Rb. Our semiquantitative RT-PCR analysis of the PIK3CA, AKT1, FRAP1, and APS6KB1 genes in Rb samples supported this hypothesis. We suggest that known inhibitors of this pathway could be evaluated for the treatment of Rb. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：344 / 353

页数：10

共 49 条

[1] Cdc25A phosphatase: combinatorial phosphorylation, ubiquitylation and proteolysis [J].