Enzymatic synthesis of disaccharides by β-galactosidase-catalyzed glycosylation of a glycocluster

被引：2

作者：

de Santiago, R ^{[1
]}

Fernández-Mayoralas, A ^{[1
]}

García-Junceda, E ^{[1
]}

机构：

[1] CSIC, Inst Quim Organ Gen, Dept Quim Organ Biol, E-28006 Madrid, Spain

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2000年 / 11卷 / 2-3期

关键词：

enzymatic synthesis; liquid-phase synthesis; glycocluster; beta-galactosidase; glycosidase catalyzed transglycosylation;

D O I：

10.1016/S1381-1177(00)00194-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of disaccharides by galactosylation of a glucose-containing cluster has been studied using beta -galactosidases from different sources. Galactosylation of the cluster could be detected by ESI-MS. After size-exclusion chromatography of the reaction mixtures and subsequent cleavage of the linker, galactopyranosyl-glucose disaccharides were obtained with yield and regioselectivity dependent on the origin of the enzyme. The possibility of using this method for the synthesis of multivalent glycoconjugates is discussed. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：71 / 79

页数：9