Rapid and Reliable Single Nucleotide Polymorphism-Based Differentiation of Brucella Live Vaccine Strains from Field Strains

被引：30

作者：

Gopaul, Krishna K. ^{[1
]}

Sells, Jessica ^{[1
]}

Bricker, Betsy J. ^{[2
]}

Crasta, Oswald R. ^{[3
]}

Whatmore, Adrian M. ^{[1
]}

机构：

[1] Vet Labs Agcy, Dept Statutory & Exot Bacterial Dis, New Haw KT15 3NB, England

[2] USDA, Ames, IA 50010 USA

[3] Virginia Bioinformat Inst, Blacksburg, VA 24061 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 04期

关键词：

PCR ASSAY; ABORTUS S19; RB51; IDENTIFICATION; MELITENSIS; VIRULENCE; GENES; GOATS;

D O I：

10.1128/JCM.02193-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The reliable differentiation of live Brucella vaccine strains from field isolates is an important element in brucellosis control programs. We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrinsic part of, a previously described assay identifying Brucella isolates to the species level (K. K. Gopaul, C. J. Smith, M. S. Koylass, and A. M. Whatmore, BMC Microbiol. 8:86), giving a comprehensive molecular typing platform.

引用

页码：1461 / 1464

页数：4

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