Development of the custom polymeric materials specific for aflatoxin B1 and ochratoxin A for application with the ToxiQuant T1 sensor tool

被引：21

作者：

Piletska, Elena ^{[1
]}

Karim, Kal ^{[1
]}

Coker, Raymond ^{[2
]}

Piletsky, Sergey ^{[1
]}

机构：

[1] Cranfield Univ, Cranfield MK43 0AL, Beds, England

[2] Univ Greenwich, Toximet Ltd, Chatham ME4 4TB, Kent, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 16期

关键词：

Mycotoxins; Aflatoxin B1; Ochratoxin A; Computational modelling; Solid-phase extraction; MOLECULARLY IMPRINTED POLYMERS;

D O I：

10.1016/j.chroma.2009.11.091

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Two polymers were computationally designed with affinity to two of the most abundant mycotoxins aflatoxin B1 (AFB1) and ochratoxin A (OTA) for application in the ToxiQuant T1 System. The principle of quantification of AFB1 and OTA using the ToxiQuant T1 instrument comprised of a fluorimetric analysis of mycotoxins adsorbed on the polymer upon exposure to UV light. High affinity of the developed resins allowed the adsorption of both toxins as discrete bands on the top of the cartridge with detection limit as low as 1 ng quantity of mycotoxins. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：2543 / 2547

页数：5

共 11 条

[1]

[Anonymous], 1993, IARC MONOGRAPHS EVAL, V58

[2]

[Anonymous], OFFICIAL J EUROPEA L

[3]

AOAC, 1995, OFF METH AN, V2

[4]

Molecularly imprinted polymers: synthesis and characterisation [J].