Expression, crystallization and preliminary X-ray diffraction studies of recombinant Bacillus anthracis lethal factor

被引：4

作者：

Bernardi, L

Vitale, G ^{[1
]}

Montecucco, C

Musacchio, A

机构：

[1] Univ Padua, Ctr CNR Biomembrane, I-35121 Padua, Italy

[2] Univ Padua, Dipartimento Sci Biomed, I-35121 Padua, Italy

[3] European Inst Oncol, Dept Expt Oncol, Struct Biol Unit, I-20141 Milan, Italy

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444900010027

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The lethal factor (LF) produced by Bacillus anthracis is a Zn2+ dependent endopeptidase which specifically cleaves the N-terminal tail of several MAP kinase kinases (MAPKKs). The recombinant expression, purification and crystallization of LF and of an inactive mutant consisting of a single amino-acid substitution in the conserved catalytic site are reported here. Both proteins crystallize in the cubic space group I432.

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页码：1449 / 1451

页数：3

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