Self-assembly of minimal COPII cages

被引：44

作者：

Antonny, B

Gounon, P

Schekman, R

Orci, L

机构：

[1] CNRS, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France

[2] Univ Nice, Ctr Commun Microscopie Appl, F-06108 Nice, France

[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[5] Univ Geneva, Dept Morphol, CH-1211 Geneva 4, Switzerland

来源：

EMBO REPORTS | 2003年 / 4卷 / 04期

关键词：

D O I：

10.1038/sj.embor.embor812

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The small G-protein Sar1 and the cytosolic complexes Sec23/24 and Sec13/31 associate sequentially on endoplasmic reticulum membranes to form a protein coat named COPII, which drives the formation of transport vesicles. Using dynamic light scattering, we show that Sec23/24 and Sec13/31 can self-assemble in a stoichiometric manner in solution to form particles with hydrodynamic radii in the range of 40-60 nm. Self-assembly is favoured by lowering the pH, the ionic strength and/or the temperature. Electron microscopy reveals the formation of spherical particles 60-120 nm in diameter with a tight, rough mesh on their surfaces. We suggest that these stuctures, which represent a minimal COPII cage, mimic the molecular organization of the membrane-associated COPII coat.

引用

页码：419 / 424

页数：6

共 13 条

[1] ER export: public transportation by the COPII coach [J].