Extended-Spectrum Cephalosporinase in Acinetobacter baumannii

被引：64

作者：

Rodriguez-Martinez, Jose-Manuel ^{[1
,2
]}

Nordmann, Patrice ^{[1
,2
]}

Ronco, Esthel ^{[3
]}

Poirel, Laurent ^{[1
,2
]}

机构：

[1] Univ Paris 11, Hop Bicetre, F-94275 K Bicetre, France

[2] AP HP, Fac Med, INSERM, Serv Bacteriol Virol,Emerging Resistance Antibiot, F-94275 K Bicetre, France

[3] Hop Raymond Poincare, AP HP, Fac Med Paris Ouest, Microbiol Serv, F-92380 Garches, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 08期

关键词：

C BETA-LACTAMASE; BIOCHEMICAL-CHARACTERIZATION; RESISTANCE; INSERTION;

D O I：

10.1128/AAC.00050-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An AmpC-type beta-lactamase conferring high-level resistance to expanded-spectrum cephalosporins and monobactams was characterized from an Acinetobacter baumannii clinical isolate. This class C beta-lactamase (named ADC-33) possessed a Pro210Arg substitution together with a duplication of an Ala residue at position 215 (inside the Omega-loop) compared to a reference AmpC cephalosporinase from A. baumannii. ADC-33 hydrolyzed ceftazidime, cefepime, and aztreonam at high levels, which allows the classification of this enzyme as an extended-spectrum AmpC (ESAC). Site-directed mutagenesis confirmed the role of both substitutions in its ESAC property.

引用

收藏

页码：3484 / 3488

页数：5

相关论文

共 23 条

[1]

[Anonymous], 2010, PERFORMANCE STANDARD

[2] Cloning, nucleotide sequencing, and analysis of the gene encoding an AmpC β-lactamase in Acinetobacter baumannii [J].

Bou, G ;

Martínez-Beltrán, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (02) :428-432

[3] Correlation of antimicrobial resistance with β-lactamases, the OmpA-Like porin, and efflux pumps in clinical isolates of Acinetobacter baumannii endemic to New York city [J].

Bratu, Simona ;

Landman, David ;

Martin, Don Antonio ;

Georgescu, Claudiu ;

Quale, John .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (09) :2999-3005

[4] A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].

BUSH, K ;

JACOBY, GA ;

MEDEIROS, AA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233

[5] Nosocomial bacteremia due to Acinetobacter baumannii:: epidemiology, clinical features and treatment [J].

Cisneros, JM ;

Rodríguez-Baño, J .

CLINICAL MICROBIOLOGY AND INFECTION, 2002, 8 (11) :687-693

[6] Structure of the extended-spectrum class C β-lactamase of Enterobacter cloacae GC1, a natural mutant with a tandem tripeptide insertion [J].

Crichlow, GV ;

Kuzin, AP ;

Nukaga, M ;

Mayama, K ;

Sawai, T ;

Knox, JR .

BIOCHEMISTRY, 1999, 38 (32) :10256-10261

[7] Comparative genomics of multidrug resistance in Acinetobacter baumannii [J].

Fournier, Pierre-Edouard ;

Vallenet, David ;

Barbe, Valerie ;

Audic, Stephane ;

Ogata, Hiroyuki ;

Poirel, Laurent ;

Richet, Herve ;

Robert, Catherine ;

Mangenot, Sophie ;

Abergel, Chantal ;

Nordmann, Patrice ;

Weissenbach, Jean ;

Raoult, Didier ;

Claverie, Jean-Michel .

PLOS GENETICS, 2006, 2 (01) :62-72

[8]

Hancock REW, 1996, J CHEMOTHERAPY, V8, P31

[9] Cephalosporinase over-expression resulting from insertion of ISAba1 in Acinetobacter baumannii [J].

Héritier, C ;

Poirel, L ;

Nordmann, P .

CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 (02) :123-130

[10] Identification of a new allelic variant of the Acinetobacter baumannii cephalosporinase, ADC-7 β-lactamase:: Defining a unique family of class C enzymes [J].

Hujer, KM ;

Hamza, NS ;

Hujer, AM ;

Perez, F ;

Helfand, MS ;

Bethel, CR ;

Thomson, JM ;

Anderson, VE ;

Barlow, M ;

Rice, LB ;

Tenover, FC ;

Bonomo, RA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (07) :2941-2948