Determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry

被引：9

作者：

Kage, S

Kudo, K

Ikeda, H

Tsujita, A

Ikeda, N

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Dept Forens Pathol & Sci, Higashi Ku, Fukuoka 8128582, Japan

[2] Fukuoka Prefectural Police Headquarters, Lab Forens Sci, Hakata Ku, Fukuoka 8128576, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 817卷 / 02期

关键词：

bromide; methyl bromide; toxicology; gas poisoning; metabolite; gas chromatography-mass spectrometry;

D O I：

10.1016/j.jchromb.2004.12.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We devised a sensitive and simple method for determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry. Bromide was alkylated with pentafluorobenzyl p-toluenesulphonate in the mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed using gas chromatography-mass spectrometry with the positive-ion El mode. The lower limit of detection for the compound was 1 mg/l. The calibration curve for bromide was linear over the concentration range from 2 to 100 mg/l. With use of this method, levels of bromide in whole blood and urine were determined in cases of poisoning by inhaled brominated hydrocarbons. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：335 / 339

页数：5

共 24 条

[1] DETERMINATION OF IODINE AND BROMINE IN PLASMA AND URINE BY INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY [J].