Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance

被引:198
作者
Kieboom, J
Dennis, JJ
de Bont, JAM
Zylstra, GJ
机构
[1] Rutgers State Univ, Cook Coll, Ctr Agr Mol Biol, New Brunswick, NJ 08903 USA
[2] Agr Univ Wageningen, Dept Food Sci, Div Ind Microbiol, NL-6700 EV Wageningen, Netherlands
关键词
D O I
10.1074/jbc.273.1.85
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacteria able to grow in aqueous:organic two-phase systems have evolved resistance mechanisms to the toxic effects of solvents. One such mechanism is the active efflux of solvents from the cell, preserving the integrity of the cell interior, Pseudomonas putida S12 is resistant to a wide variety of normally detrimental solvents due to the action of such an efflux pump. The genes for this solvent efflux pump were cloned from P. putida S12 and their nucleotide sequence determined. The deduced amino acid sequences encoded by the three genes involved show a striking resemblance to proteins known to be involved in proton-dependent multidrug efflux systems. Transfer of the genes for the solvent efflux pump to solvent-sensitive P. putida strains results in the acquisition of solvent resistance. This opens up the possibilities of using the solvent efflux system to construct bacterial strains capable of performing biocatalytic transformations of insoluble substrates in two-phase aqueous:organic medium.
引用
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页码:85 / 91
页数:7
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