A novel bioartificial liver with culture of porcine hepatocyte aggregates under simulated microgravity

被引：30

作者：

Hochleitner, B

Hengster, P

Duo, L

Bucher, H

Klima, G

Margreiter, R

机构：

[1] Univ Innsbruck Hosp, Dept Gen & Transplant Surg, A-6020 Innsbruck, Austria

[2] Univ Innsbruck Hosp, Dept Histol & Mol Cell Biol, A-6020 Innsbruck, Austria

来源：

ARTIFICIAL ORGANS | 2005年 / 29卷 / 01期

关键词：

aggregates; bioreactor; liver support; hybrid liver; microgravity-based bioreactors; rotating-wall vessel;

D O I：

10.1111/j.1525-1594.2004.29014.x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

An extracorporeal bioartificial liver device could provide vital support to patients suffering from acute liver failure. We designed a novel, customized bioreactor for use as a bioartificial liver (patent pending). The Innsbruck Bioartificial Liver (IBAL) contains aggregates of porcine hepatocytes grown under simulated microgravity. The culture vessel rotates around its longitudinal axis and is perfused by two independent circuits. The circuit responsible for exchange of plasma components with the patient consists of a dialysis tube winding spirally around the internal wall of the culture vessel. IBAL was evaluated in vitro. Viability tests showed sufficient viability of hepatocytes for up to 10 days. Cytologic examination of samples from the bioreactor showed liver cell aggregates. These were also examined by electron microscopy. A number of biochemical parameters were analyzed. In conclusion, cell culture is possible for at least 10 days in the IBAL system, organoid hepatocyte aggregates are formed and synthetic activity of the hepatocytes was demonstrated.

引用

页码：58 / 66

页数：9

共 30 条

[1] High level benzodiazepine and ammonia clearance by flat membrane bioreactors with porcine liver cells [J].