Introducing genetically encoded aldehydes into proteins

被引：336

作者：

Carrico, Isaac S.

Carlson, Brian L.

Bertozzi, Carolyn R.

机构：

[1] Univ Calif Berkeley, Dept Chem, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

NATURE CHEMICAL BIOLOGY | 2007年 / 3卷 / 06期

关键词：

D O I：

10.1038/nchembio878

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.

引用

页码：321 / 322

页数：2

共 15 条

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