Fate of prions in soil: Degradation of recombinant prion in aqueous extracts from soil and casts of two earthworm species

被引：7

作者：

Nechitaylo, Taras Y. ^{[1
]}

Timmis, Kenneth N. ^{[1
,7
]}

Byzov, Boris A. ^{[2
]}

Kurakov, Alexander V. ^{[3
]}

Belogolova, Elena ^{[1
]}

Jones, David L. ^{[4
]}

Ferrer, Manuel ^{[5
]}

Golyshin, Peter N. ^{[1
,6
]}

机构：

[1] Helmholtz Ctr Infect Res, Environm Microbiol Lab, D-38124 Braunschweig, Germany

[2] Moscow MV Lomonosov State Univ, Dept Soil Sci, Fac Soil Sci, Moscow 117234, Russia

[3] Moscow MV Lomonosov State Univ, Int Ctr Biotechnol, Moscow, Russia

[4] Bangor Univ, Sch Environm & Nat Resources, Bangor LL57 2UW, Gwynedd, Wales

[5] CSIC, Inst Catalysis, Lab Enzyme Discovery, E-28049 Madrid, Spain

[6] Bangor Univ, Sch Biol Sci, Bangor LL57 2DG, Gwynedd, Wales

[7] Tech Univ Carolo Wilhelmina Braunschweig, Inst Microbiol, Bioctr, D-38106 Braunschweig, Germany

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 2010年 / 42卷 / 07期

基金：

俄罗斯基础研究基金会;

关键词：

TSE; Prion protein; Degradation; Proteases; Earthworm; Bacteria; Fungi; PROTEIN PRPSC; GASTROINTESTINAL MICROBIOTA; NEURONAL CELLS; SCRAPIE; ADSORPTION; DECOMPOSITION; BACTERIA; STRAIN; SHEEP;

D O I：

10.1016/j.soilbio.2010.03.004

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Prions represent the active agent in transmissible spongiform encephalopathy (TSE) diseases and can remain infective to mammals even after prolonged periods in soil. The influence of mesofauna on prion dispersal and degradation in soil, however, remains unknown. In this study the effect of earthworms on the retention/dissemination of TSEs in soil was evaluated using a model recombinant prion protein (recPrP) and aqueous extracts from soil and fresh casts of two earthworm species, Lumbricus terrestris and Aporrectodea caliginosa. Our results showed that earthworm gut-derived enzymes did not enhance the degradation of recPrP in comparison to soil, even though non-prion related proteolytic activity was higher in fresh worm excrements than in soil samples. Complete degradation of recPrP occurred in the aqueous extracts from all samples within up to 6 days at +15 degrees C. The proteolytic enzymes responsible for degrading recPrP were inhibited by aprotinin and leupeptin and studies in pure cultures suggested these were most probably of soil microbial origin. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1168 / 1171

页数：4

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