Detection of t(11;18)(q21;q21) by interphase fluorescence in situ hybridization using API2 and MLT specific probes

被引:79
作者
Dierlamm, J
Baens, M
Stefanova-Ouzounova, M
Hinz, K
Wlodarska, I
Maes, B
Steyls, A
Driessen, A
Verhoef, G
Gaulard, P
Hagemeijer, A
Hossfeld, DK
De Wof-Peeters, C
Marynen, P
机构
[1] Catholic Univ Louvain, Ctr Human Genet, Human Genome Lab, B-3000 Louvain, Belgium
[2] CHU Henri Mondor, Dept Pathol, F-94010 Creteil, France
[3] Catholic Univ Louvain, Dept Pathol, B-3000 Louvain, Belgium
[4] Catholic Univ Louvain, Dept Hematol, B-3000 Louvain, Belgium
[5] Catholic Univ Louvain VIB, B-3000 Louvain, Belgium
[6] Univ Hamburg, Hosp Eppendorf, Dept Hematol & Oncol, D-20246 Hamburg, Germany
关键词
D O I
10.1182/blood.V96.6.2215.h8002215_2215_2218
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The translocation of chromosome 11, long arm, region 2, band 1, to chromosome 18, long arm, region 2, band 1 (t(11;18)(q21;q21)) represents a recurrent chromosomal abnormality in extranodal marginal zone B-cell lymphoma (MZBCL) of mucosa-associated lymphoid tissue (MALT) type and leads to a fusion of the apoptosis inhibitor-2 (API2) gene on chromosome 11 and the MALT lymphoma-associated translocation (MLT) gene on chromosome 18. A 2-color fluorescence in situ hybridization (FISH) assay, which can be used for the detection of t(11;18) in interphase nuclei and metaphase chromosomes on fresh and archival tumor tissue, was developed. The P1 artificial chromosome (PAC) clone located immediately telomeric to the MLT gene and the PAC clone spanning the API2 gene were differentially labeled and used to visualize the derivative chromosome II resulting from t(11;18), as evident by the overlapping or juxtaposed red and green fluorescent signals. The assay was applied to interphase nuclei of 20 cases with nonmalignant conditions and 122 B-cell non-Hodgkin's lymphomas (NHLs), The latter group comprised 20 cases of nodal follicle center cell lymphoma and diffuse large B-cell NHL, 10 cases of gastric diffuse large B-cell lymphoma, 10 cases of hairy cell leukemia, and 82 cases of MZBCL (41 extranodal from various locations, 19 nodal, and 22 splenic MZBCL) including 35 cases with an abnormal karyotype, 2 of which revealed t(11;18). By interphase FISH, t(11;18) was detected in 8 gastrointestinal low-grade MALT-type lymphomas including the 2 cytogenetically t(11;18)(+) cases. In the 8 t(11;18)(+) cases, the FISH results were confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) using API2 and MLT specific primers. Our results indicate that t(11;18)(q21;q21) specifically characterizes a subgroup of low grade MZBCL of the MALT-type and that the FISH assay described here is a highly specific and rapid test for the detection of this translocation. (Blood, 2000;96:2215-2218) (C) 2000 by The American Society of Hematology.
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页码:2215 / 2218
页数:4
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