Capillary isoelectric focusing

被引：107

作者：

Rodriguez-Diaz, R

Wehr, T

Zhu, MD

机构：

[1] Bio Rad Labs, Hercules, CA 94547 USA

[2] Chiron Corp, Emeryville, CA USA

来源：

ELECTROPHORESIS | 1997年 / 18卷 / 12-13期

基金：

美国国家航空航天局; 美国国家科学基金会;

关键词：

capillary isoelectric focusing;

D O I：

10.1002/elps.1150181204

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a capillary format with on-tube detection and automated analysis. Recent advances in the technique provide improved resolution, reproducibility and reliability. This review summarizes improvements in sample preparation, capillary selection, and focusing and zone mobilization procedures which have helped CIEF become a more robust analytical method. New applications are reviewed, in particular the use of CIEF in analysis of biopharmaceutical products.

引用

收藏

页码：2134 / 2144

页数：11

相关论文

共 54 条

[11] THEORETICAL AND EXPERIMENTAL-STUDY OF HIGH-PERFORMANCE ELECTROPHORETIC MOBILIZATION OF ISOELECTRICALLY FOCUSED PROTEIN ZONES [J].

HJERTEN, S ;

LIAO, JL ;

YAO, K .

JOURNAL OF CHROMATOGRAPHY, 1987, 387 :127-138

[12] ISOELECTRIC-FOCUSING OF PROTEINS IN CAPILLARY ELECTROPHORESIS WITH PRESSURE-DRIVEN MOBILIZATION [J].

HUANG, TL ;

SHIEH, PCH ;

COOKE, N .

CHROMATOGRAPHIA, 1994, 39 (9-10) :543-548

[13] Capillary isoelectric focusing and sodium dodecyl sulfate capillary gel electrophoresis of recombinant humanized monoclonal antibody HER2 [J].

Hunt, G ;

Moorhouse, KG ;

Chen, AB .

JOURNAL OF CHROMATOGRAPHY A, 1996, 744 (1-2) :295-301

[14] FAST AND HIGH-RESOLUTION ANALYSIS OF HUMAN-SERUM TRANSFERRIN BY HIGH-PERFORMANCE ISOELECTRIC-FOCUSING IN CAPILLARIES [J].

KILAR, F ;

HJERTEN, S .

ELECTROPHORESIS, 1989, 10 (01) :23-29

[15] Non-native capillary isoelectric focusing for the analysis of the microheterogeneity of glycoproteins [J].

Kubach, J ;

Grimm, R .

JOURNAL OF CHROMATOGRAPHY A, 1996, 737 (02) :281-289

[16]

Kundu S, 1995, J CAPILLARY ELECTROP, V2, P273

[17]

Mazzeo Jeff R., 1992, Methods (Orlando), V4, P205, DOI 10.1016/1046-2023(92)90035-7

[18] CAPILLARY ISOELECTRIC-FOCUSING OF PROTEINS IN UNCOATED FUSED-SILICA CAPILLARIES USING POLYMERIC ADDITIVES [J].

MAZZEO, JR ;

KRULL, IS .

ANALYTICAL CHEMISTRY, 1991, 63 (24) :2852-2857

[19] PEPTIDE-MAPPING USING EOF-DRIVEN CAPILLARY ISOELECTRIC-FOCUSING [J].

MAZZEO, JR ;

MARTINEAU, JA ;

KRULL, IS .

ANALYTICAL BIOCHEMISTRY, 1993, 208 (02) :323-329

[20] Dispersion effects accompanying pressurized zone mobilisation in capillary isoelectric focusing of proteins [J].

Minarik, M ;

Groiss, F ;

Gas, B ;

Blaas, D ;

Kenndler, E .

JOURNAL OF CHROMATOGRAPHY A, 1996, 738 (01) :123-128

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