Tissue-specific developmental requirements of Drosophila tyrosine hydroxylase isoforms

被引:27
作者
Friggi-Grelin, F [1 ]
Iché, M [1 ]
Birman, S [1 ]
机构
[1] Dev Biol Inst Marseille, Lab Genet & Physiol Dev, F-13288 Marseille 9, France
关键词
dopamine; tyrosine hydroxylase; nervous system; hypoderm; development; Drosophila melanogaster;
D O I
10.1002/gene.10178
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Drosophila tyrosine hydroxylase (DTH) is a key enzyme in dopamine (DA) biosynthesis, which is expressed in neural and hypodermal DA-synthesizing cells. We previously reported that two DTH isoforms are produced in flies through tissue-specific alternative splicing that show distinct regulatory properties. We have now selectively expressed each DTH isoform in vivo in a pale (pie, i.e., DTH-deficient) mutant background. We show that the embryonic lethality of pie can be rescued by expression of the hypodermal, but not the neural, DTH isoform in all DA cells, indicating that the hypoderm- isoform is absolutely required for cuticle biosynthesis and survival in Drosophila. In addition, we report new observations on the consequences of DTH overexpression in the CNS and hypoderm. Our results provide evidence that tissue-specific alternative splicing of the DTH gene is a vital process in Drosophila development. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:175 / 184
页数:10
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