Adaptation of the fluid percussion injury model to the mouse

被引:106
作者
Carbonell, WS [1 ]
Maris, DO [1 ]
McCall, T [1 ]
Grady, MS [1 ]
机构
[1] Univ Washington, Sch Med, Dept Neurol Surg, Seattle, WA 98104 USA
关键词
axonal degeneration; C57B1/6; cell death; gliosis; mice; Morris water maze; traumatic brain injury;
D O I
10.1089/neu.1998.15.217
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Fluid percussion injury (FPI) is a well-characterized experimental model of traumatic brain injury (TBI) in the rat. Many pathophysiologic consequences and mechanisms of recovery after TBI rely on neurochemical pathways that can be examined in genetically altered mice. Therefore, FPI applied to mice may be a useful experimental tool to investigate TBI at the molecular level. In the present study, we establish FPI as a viable model of TBI in the mouse by characterizing acute neurological, histopathological, and behavioral changes. Right-sided parasagittal FPI or sham treatment was administered in male C57BL/6 mice. Acute neurological evaluation revealed righting reflexes in the injured animals (p < 0.001). Deficits in spatial learning and memory were observed in the Morris water maze (MWM) 5 and 6 days after injury. A novel MWM data analysis protocol is described. The injured group (n = 18) demonstrated impaired performance in the MWM during acquisition (p < 0.05) and probe trials (p < 0.025) compared to sham animals (n = 16). At 7 days postinjury, glial fibrillary acidic protein immunohistochemistry revealed intense cortical, callosal, and hippocampal gliosis. The modified Gallyas silver degeneration stain consistently labeled cell bodies and terminals throughout the ipsilateral cortex, axons in the gray matter-white matter interface above the corpus callosum and within the corpus callosum bilaterally, and terminals and fibers in the thalamus bilaterally. Additionally, the mouse FPI model described is immediately employable in labs already using the FPI rat model with no modifications to a pre-existing PPI apparatus.
引用
收藏
页码:217 / 229
页数:13
相关论文
共 58 条
[1]   HIPPOCAMPAL MESSY FIBERS AND SWIMMING NAVIGATION IN MICE - CORRELATIONS WITH SIZE AND LEFT-RIGHT ASYMMETRIES [J].
BERNASCONIGUASTALLA, S ;
WOLFER, DP ;
LIPP, HP .
HIPPOCAMPUS, 1994, 4 (01) :53-63
[2]   An experimental model of closed head injury in mice: Pathophysiology, histopathology, and cognitive deficits [J].
Chen, Y ;
Constantini, S ;
Trembovler, V ;
Weinstock, M ;
Shohami, E .
JOURNAL OF NEUROTRAUMA, 1996, 13 (10) :557-568
[3]   A proposed test battery and constellations of specific behavioral paradigms to investigate the behavioral phenotypes of transgenic and knockout mice [J].
Crawley, JN ;
Paylor, R .
HORMONES AND BEHAVIOR, 1997, 31 (03) :197-211
[4]   Behavioral phenotypes of inbred mouse strains: implications and recommendations for molecular studies [J].
Crawley, JN ;
Belknap, JK ;
Collins, A ;
Crabbe, JC ;
Frankel, W ;
Henderson, N ;
Hitzemann, RJ ;
Maxson, SC ;
Miner, LL ;
Silva, AJ ;
Wehner, JM ;
WynshawBoris, A ;
Paylor, R .
PSYCHOPHARMACOLOGY, 1997, 132 (02) :107-124
[5]  
CRAWLEY JN, 1996, TRENDS NEUROSCI, V19, P186
[6]   ATTENUATION OF P53 EXPRESSION PROTECTS AGAINST FOCAL ISCHEMIC DAMAGE IN TRANSGENIC MICE [J].
CRUMRINE, RC ;
THOMAS, AL ;
MORGAN, PF .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1994, 14 (06) :887-891
[7]  
DAMBROSIO R, IN PRESS BRAIN RES
[8]   A FLUID PERCUSSION MODEL OF EXPERIMENTAL BRAIN INJURY IN THE RAT [J].
DIXON, CE ;
LYETH, BG ;
POVLISHOCK, JT ;
FINDLING, RL ;
HAMM, RJ ;
MARMAROU, A ;
YOUNG, HF ;
HAYES, RL .
JOURNAL OF NEUROSURGERY, 1987, 67 (01) :110-119
[9]  
DIXON CE, 1991, J NEUROSCI METH, V39, P253
[10]   DIFFERENCES IN BRAIN 5-HT TRANSPORTER DISSOCIATION RATES AMONG ANIMAL SPECIES [J].
ERREBOE, I ;
PLENGE, P ;
MELLERUP, ET .
PHARMACOLOGY & TOXICOLOGY, 1995, 76 (06) :376-379