Genome-scale models of microbial cells: Evaluating the consequences of constraints

被引:727
作者
Price, ND [1 ]
Reed, JL [1 ]
Palsson, BO [1 ]
机构
[1] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
D O I
10.1038/nrmicro1023
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microbial cells operate under governing constraints that limit their range of possible functions. With the availability of annotated genome sequences, it has become possible to reconstruct genome-scale biochemical reaction networks for microorganisms. The imposition of governing constraints on a reconstructed biochemical network leads to the definition of achievable cellular functions. In recent years, a substantial and growing toolbox of computational analysis methods has been developed to study the characteristics and capabilities of microorganisms using a constraint-based reconstruction and analysis (COBRA) approach. This approach provides a biochemically and genetically consistent framework for the generation of hypotheses and the testing of functions of microbial cells.
引用
收藏
页码:886 / 897
页数:12
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