Analysis of estrogen receptor α signaling complex at the plasma membrane

被引：22

作者：

Azuma, K

Horie, K

Inoue, S

Ouchi, Y

Sakai, R

机构：

[1] Natl Canc Ctr, Div Growth Factor, Inst Res, Chuo Ku, Tokyo 1040045, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Geriatr Med, Bunkyo Ku, Tokyo 1138655, Japan

[3] Saitama Med Sch, Res Ctr Genomic Med, Div Gene Regulat & Signal Transduct, Hidaka, Saitama 3501241, Japan

来源：

FEBS LETTERS | 2004年 / 577卷 / 03期

关键词：

estrogen receptor; non-genomic action; breast cancer; tubulin; heat shock protein 70; tyrosine phosphorylation;

D O I：

10.1016/j.febslet.2004.10.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is accumulating evidence that the estrogen receptor (ER) can transduce specific signals at the plasma membrane. We tried to clarify the biological function of ER as a signaling molecule by identifying proteins that interact with the membrane-localized ER. The activation function 1 and 2 (AF-1 and AF-2) domains of ERalpha with or without the membrane-targeting sequence were stably expressed in the breast cancer cell line, MCF-7. The level of tyrosine phosphorylation of AF-2 was significantly elevated by the membrane localization. By massspectrometry analysis, alpha- and beta-tubulins and heat shock protein 70 were identified as the AF-l-associated proteins. Of these, tubulins are associated only with membrane-targeted AF-1. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：339 / 344

页数：6

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