Analysis of estrogen receptor α signaling complex at the plasma membrane

被引：22

作者：

Azuma, K

Horie, K

Inoue, S

Ouchi, Y

Sakai, R

机构：

[1] Natl Canc Ctr, Div Growth Factor, Inst Res, Chuo Ku, Tokyo 1040045, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Geriatr Med, Bunkyo Ku, Tokyo 1138655, Japan

[3] Saitama Med Sch, Res Ctr Genomic Med, Div Gene Regulat & Signal Transduct, Hidaka, Saitama 3501241, Japan

来源：

FEBS LETTERS | 2004年 / 577卷 / 03期

关键词：

estrogen receptor; non-genomic action; breast cancer; tubulin; heat shock protein 70; tyrosine phosphorylation;

D O I：

10.1016/j.febslet.2004.10.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is accumulating evidence that the estrogen receptor (ER) can transduce specific signals at the plasma membrane. We tried to clarify the biological function of ER as a signaling molecule by identifying proteins that interact with the membrane-localized ER. The activation function 1 and 2 (AF-1 and AF-2) domains of ERalpha with or without the membrane-targeting sequence were stably expressed in the breast cancer cell line, MCF-7. The level of tyrosine phosphorylation of AF-2 was significantly elevated by the membrane localization. By massspectrometry analysis, alpha- and beta-tubulins and heat shock protein 70 were identified as the AF-l-associated proteins. Of these, tubulins are associated only with membrane-targeted AF-1. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：339 / 344

页数：6

相关论文

共 26 条

[11] INVOLVEMENT OF PP60(C-SRC) WITH 2 MAJOR SIGNALING PATHWAYS IN HUMAN BREAST-CANCER [J].

LUTTRELL, DK ;

LEE, A ;

LANSING, TJ ;

CROSBY, RM ;

JUNG, KD ;

WILLARD, D ;

LUTHER, M ;

RODRIGUEZ, M ;

BERMAN, J ;

GILMER, TM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (01) :83-87

[12] THE NUCLEAR RECEPTOR SUPERFAMILY - THE 2ND DECADE [J].

MANGELSDORF, DJ ;

THUMMEL, C ;

BEATO, M ;

HERRLICH, P ;

SCHUTZ, G ;

UMESONO, K ;

BLUMBERG, B ;

KASTNER, P ;

MARK, M ;

CHAMBON, P ;

EVANS, RM .

CELL, 1995, 83 (06) :835-839

[13] The Hsc70 co-chaperone CHIP targets immature CFTR for proteasomal degradation [J].

Meacham, GC ;

Patterson, C ;

Zhang, WY ;

Younger, JM ;

Cyr, DM .

NATURE CELL BIOLOGY, 2001, 3 (01) :100-105

[14] Steroid-induced androgen receptor-oestradiol receptor β-Src complex triggers prostate cancer cell proliferation [J].

Migliaccio, A ;

Castoria, G ;

Di Domenico, M ;

de Falco, A ;

Bilancio, A ;

Lombardi, M ;

Barone, MV ;

Ametrano, D ;

Zannini, MS ;

Abbondanza, C ;

Auricchio, F .

EMBO JOURNAL, 2000, 19 (20) :5406-5417

[15]

MUTHUSWAMY SK, 1995, ONCOGENE, V11, P271

[16] EPIDERMAL-GROWTH-FACTOR-DEPENDENT ACTIVATION OF THE SRC-FAMILY KINASES [J].

OSHEROV, N ;

LEVITZKI, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 225 (03) :1047-1053

[17] Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex [J].

Rachez, C ;

Lemon, BD ;

Suldan, Z ;

Bromleigh, V ;

Gamble, M ;

Näär, AM ;

Erdjument-Bromage, H ;

Tempst, P ;

Freedman, LP .

NATURE, 1999, 398 (6730) :824-828

[18] Cyclic, proteasome-mediated turnover of unliganded and liganded ERα on responsive promoters is an integral feature of estrogen signaling [J].

Reid, G ;

Hübner, MR ;

Métivier, R ;

Brand, H ;

Denger, S ;

Manu, D ;

Beaudouin, J ;

Ellenberg, J ;

Gannon, F .

MOLECULAR CELL, 2003, 11 (03) :695-707

[19] Interaction of oestrogen receptor with the regulatory subunit of phosphatidylinositol-3-OH kinase [J].

Simoncini, T ;

Hafezl-Moghadam, A ;

Brazil, DP ;

Ley, K ;

Chin, WW ;

Liao, JK .

NATURE, 2000, 407 (6803) :538-541

[20] Linkage of rapid estrogen action to MAPK activation by ERα-Shc association and Shc pathway activation [J].

Song, RXD ;

McPherson, RA ;

Adam, L ;

Bao, YD ;

Shupnik, M ;

Kumar, R ;

Santen, RJ .

MOLECULAR ENDOCRINOLOGY, 2002, 16 (01) :116-127