Time-gated fluorescence spectroscopy improves endoscopic detection of low-grade dysplasia in ulcerative colitis

Background: Dysplasia in ulcerative colitis is frequently missed with 4-quadrant biopsies. An experimental setup recording delayed fluorescence spectra simultaneously With white light endoscopy was recently, developed. Objective: We compared detection of invisible flat intraepithelial neoplasia with protoporphyrin IX fluorescence and standard 4-quadrant biopsies. Design: Prospective, crossover design without randomization of the order of procedures. Setting: Gastroenterology Department, Humboldt University, Charite, Berlin, Germany. Patients: Forty-two patients with extensive ulcerative colitis of more than 10 years' duration were included. Interventions: Colonoscopy with 4-quadrant biopsies and targeted biopsies of macroscopic lesions and time-gated fluorescence-guided colonoscopy were performed 2 weeks apart by 2 blinded endoscopists. Three independent pathologists examined the biopsy specimens. Main Outcome Measurements: The primary Outcome criterion was detection rate of invisible flat intraepithelial neoplasia. Results: Invisible flat intraepithelial neoplasia was detected in 3 (7%) patients by white light 4-quadrant biopsies and in 10 (24%) patients by fluorescence-guided endoscopy (P = .02). The sensitivity and specificity for differentiating patients with and without dysplasia were 100% and 81%, respectively. Dysplastic and nondysplastic mucosa Could be discriminated with a sensitivity and specificity of 73% and 81%, respectively. Limitations: The trial was not randomized. Conclusion: The detection rate of intraepithelial neoplasia in patients with ulcerative colitis can be improved 1 by fluorescence-guided colonoscopy. (Gastrointest Endosc 2010;71:312-8.)