Motions of single molecules and proteins in trehalose glass

被引：58

作者：

Mei, E

Tang, JY

Vanderkooi, JM

Hochstrasser, RM ^{[1
]}

机构：

[1] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 09期

关键词：

D O I：

10.1021/ja021197t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The fluorescence intensity-time records of individual metal-free porphyrin cytochrome-c and Zn porphyrin cytochrome-c molecules whose translational motions are restricted by encapsulation in trehalose are examined by single-molecule spectroscopy by means of a two-channel confocal microscope that records transient fluorescence signals in two orthogonal polarization directions. Large angular motions often occur on time scales ranging to many seconds. Measurements of the photobleaching time distributions indicate that the trehalose glass restricts the accessibilty of the fluorescent molecules to oxygen.

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页码：2730 / 2735

页数：6

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