Stable-isotope dilution LC-MS for quantitative biomarker analysis

被引：158

作者：

Ciccimaro, Eugene ^{[3
]}

Blair, Ian A. ^{[1
,2
]}

机构：

[1] Univ Penn, Dept Pharmacol, Sch Med, Ctr Excellence Environm Toxicol, 421 Curie Blvd, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Pharmacol, Sch Med, Ctr Canc Pharmacol, Philadelphia, PA 19104 USA

[3] Thermo Fisher Sci, Somerset, NJ 08873 USA

来源：

BIOANALYSIS | 2010年 / 2卷 / 02期

关键词：

OXIDATIVE DNA-DAMAGE; TANDEM MASS-SPECTROMETRY; MONOCYTE CHEMOATTRACTANT PROTEIN-1; CHIRAL LIPIDOMICS ANALYSIS; PROSTATE-SPECIFIC ANTIGEN; LIQUID-CHROMATOGRAPHY; ELECTROSPRAY-IONIZATION; ABSOLUTE QUANTIFICATION; BREAST-CANCER; HUMAN SERUM;

D O I：

10.4155/BIO.09.185

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability to conduct validated analyses of biomarkers is critically important in order to establish the sensitivity and selectivity of the biomarker in identifying a particular disease. The use of stable-isotope dilution (SID) methodology in combination with LC-MS/MS provides the highest possible analytical specificity for quantitative determinations. This methodology is now widely used in the discovery and validation of putative exposure and disease biomarkers. This review will describe the application of SID LC-MS methodology for the analysis of small-molecule and protein biomarkers. It will also discuss potential future directions for the use of this methodology for rigorous biomarker analysis.

引用

页码：311 / 341

页数：31

共 248 条

[1] Coupling immunoaffinity techniques with MS for quantitative analysis of low-abundance protein biomarkers [J].

Ackermann, Bradley L. ;

Berna, Michael J. .

EXPERT REVIEW OF PROTEOMICS, 2007, 4 (02) :175-186

[2] Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma [J].

Addona, Terri A. ;

Abbatiello, Susan E. ;

Schilling, Birgit ;

Skates, Steven J. ;

Mani, D. R. ;

Bunk, David M. ;

Spiegelman, Clifford H. ;

Zimmerman, Lisa J. ;

Ham, Amy-Joan L. ;

Keshishian, Hasmik ;

Hall, Steven C. ;

Allen, Simon ;

Blackman, Ronald K. ;

Borchers, Christoph H. ;

Buck, Charles ;

Cardasis, Helene L. ;

Cusack, Michael P. ;

Dodder, Nathan G. ;

Gibson, Bradford W. ;

Held, Jason M. ;

Hiltke, Tara ;

Jackson, Angela ;

Johansen, Eric B. ;

Kinsinger, Christopher R. ;

Li, Jing ;

Mesri, Mehdi ;

Neubert, Thomas A. ;

Niles, Richard K. ;

Pulsipher, Trenton C. ;

Ransohoff, David ;

Rodriguez, Henry ;

Rudnick, Paul A. ;

Smith, Derek ;

Tabb, David L. ;

Tegeler, Tony J. ;

Variyath, Asokan M. ;

Vega-Montoto, Lorenzo J. ;

Wahlander, Asa ;

Waldemarson, Sofia ;

Wang, Mu ;

Whiteaker, Jeffrey R. ;

Zhao, Lei ;

Anderson, N. Leigh ;

Fisher, Susan J. ;

Liebler, Daniel C. ;

Paulovich, Amanda G. ;

Regnier, Fred E. ;

Tempst, Paul ;

Carr, Steven A. .

NATURE BIOTECHNOLOGY, 2009, 27 (07) :633-U85

[3] Mass spectrometry-based proteomics [J].

Aebersold, R ;

Mann, M .

NATURE, 2003, 422 (6928) :198-207

[4] Quantitative Analysis of an Aberrant Glycoform of TIMP1 from Colon Cancer Serum by L-PHA-Enrichment and SISCAPA with MRM Mass Spectrometry [J].

Ahn, Yeong Hee ;

Lee, Ji Yeon ;

Lee, Ju Yeon ;

Kim, Yong-Sam ;

Ko, Jeong Heon ;

Yoo, Jong Shin .

JOURNAL OF PROTEOME RESEARCH, 2009, 8 (09) :4216-4224

[5] OXIDANTS, ANTIOXIDANTS, AND THE DEGENERATIVE DISEASES OF AGING [J].

AMES, BN ;

SHIGENAGA, MK ;

HAGEN, TM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :7915-7922

[6] Chemical toxicology of reactive intermediates formed by the glutathione-dependent bioactivation of halogen-containing compounds [J].

Anders, M. W. .

CHEMICAL RESEARCH IN TOXICOLOGY, 2008, 21 (01) :145-159

[7] Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins [J].

Anderson, L ;

Hunter, CL .

MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (04) :573-588

[8] A Human Proteome Detection and Quantitation Project [J].

Anderson, N. Leigh ;

Anderson, Norman G. ;

Pearson, Terry W. ;

Borchers, Christoph H. ;

Paulovich, Amanda G. ;

Patterson, Scott D. ;

Gillette, Michael ;

Aebersold, Ruedi ;

Carr, Steven A. .

MOLECULAR & CELLULAR PROTEOMICS, 2009, 8 (05) :883-886

[9] The human plasma proteome - History, character, and diagnostic prospects [J].

Anderson, NL ;

Anderson, NG .

MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (11) :845-867

[10] Mass spectrometric quantitation of peptides and proteins using stable isotope standards and capture by anti-peptide antibodies (SISCAPA) [J].

Anderson, NL ;

Anderson, NG ;

Haines, LR ;

Hardie, DB ;

Olafson, RW ;

Pearson, TW .

JOURNAL OF PROTEOME RESEARCH, 2004, 3 (02) :235-244

← 1 2 3 4 5 6 7 8 9 10 →