Stable-isotope dilution LC-MS for quantitative biomarker analysis

被引：158

作者：

Ciccimaro, Eugene ^{[3
]}

Blair, Ian A. ^{[1
,2
]}

机构：

[1] Univ Penn, Dept Pharmacol, Sch Med, Ctr Excellence Environm Toxicol, 421 Curie Blvd, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Pharmacol, Sch Med, Ctr Canc Pharmacol, Philadelphia, PA 19104 USA

[3] Thermo Fisher Sci, Somerset, NJ 08873 USA

来源：

BIOANALYSIS | 2010年 / 2卷 / 02期

关键词：

OXIDATIVE DNA-DAMAGE; TANDEM MASS-SPECTROMETRY; MONOCYTE CHEMOATTRACTANT PROTEIN-1; CHIRAL LIPIDOMICS ANALYSIS; PROSTATE-SPECIFIC ANTIGEN; LIQUID-CHROMATOGRAPHY; ELECTROSPRAY-IONIZATION; ABSOLUTE QUANTIFICATION; BREAST-CANCER; HUMAN SERUM;

D O I：

10.4155/BIO.09.185

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability to conduct validated analyses of biomarkers is critically important in order to establish the sensitivity and selectivity of the biomarker in identifying a particular disease. The use of stable-isotope dilution (SID) methodology in combination with LC-MS/MS provides the highest possible analytical specificity for quantitative determinations. This methodology is now widely used in the discovery and validation of putative exposure and disease biomarkers. This review will describe the application of SID LC-MS methodology for the analysis of small-molecule and protein biomarkers. It will also discuss potential future directions for the use of this methodology for rigorous biomarker analysis.

引用

页码：311 / 341

页数：31

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