Identification and characterization of microsatellites in eggplant

被引：78

作者：

Nunome, T

Suwabe, K

Iketani, H

Hirai, M

机构：

[1] NIVTS, Ano, Mie 5142392, Japan

[2] NARO, Tsukuba, Ibaraki 3058517, Japan

[3] Kyoto Prefectural Univ, Fac Agr, Kyoto 6190244, Japan

来源：

PLANT BREEDING | 2003年 / 122卷 / 03期

关键词：

Solanum melongena; microsatellites; molecular markers;

D O I：

10.1046/j.1439-0523.2003.00816.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The potential of microsatellite markers for use in genetic studies in eggplant, Solanum melongena , has been evaluated. A genomic library of eggplant was screened for GA and GT repeat motifs to isolate microsatellite clones. The frequency of each repeat motif in the eggplant genome was found to be every 3200 kb for GA repeats and every 820 kb for GT repeats. Sixty-one per cent of GT repeats were found to directly flank AT repeats. A total of 37 polymerase chain reaction (PCR) primer pairs were designed, 23 of which amplified a single product or several products. The level of microsatellite polymorphism was evaluated by using S. melongena lines and related Solanum species. Two to six alleles per primer pair were displayed in the S. melongena lines and two to 13 alleles were displayed in the Solanum relatives. Seven microsatellites showed polymorphism between parental lines of the mapping population and segregated in a codominant Mendelian manner. These microsatellite loci were distributed throughout the linkage map.

引用

页码：256 / 262

页数：7