The pig caecum model: A suitable tool to study the intestinal metabolism of flavonoids

被引:68
作者
Labib, S
Erb, A
Kraus, M
Wickert, T
Richling, E [1 ]
机构
[1] Univ Wurzburg, Lehrstuhl Lebensmittelchem, D-97074 Wurzburg, Germany
[2] FRUIT Int Fdn, Heidelberg, Germany
关键词
flavonoid metabolism; pig caecum model; polyphenols;
D O I
10.1002/mnfr.200400022
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Pig caecum was used under anaerobic conditions to metabolize flavonoids from several classes, i.e., chrysin 1, naringenin 2, quercetin 3, and hesperetin 4. Whereas chrysin 1 was not converted by the pig intestinal flora under the experimental conditions used, naringenin 2 was transformed to 3-(4-hydroxyphenyl)-propionic acid and 3-phenylpropionic acid. Quercetin 3 was metabolized to phloroglucinol, 3,4-dihydroxyphenylacetic acid, and 3,4-dihydroxytoluene. Hesperetin 4 was degraded via eriodictyol to 3-(3-hydroxyphenyl)-propionic acid and phloroglucinol. Structural elucidation of the formed metabolites was performed by high-performance liquid chromatography - diode array detection (HPLC-DAD) as well as HPLC-electrospray ionization - mass spectrometry (ESI-MS (MS)) and high resolution gas chromatography-mass spectrometry (HRGC-MS) analyses. The time course of microbial conversion of 2-4 was determined by HPLC-DAD analysis, revealing slow degradation of 2 and rapid transformation of 3 and 4. The results lead to the conclusion that the pig caecum model is a suitable ex vivo model for studying the intestinal degradation of flavonoids.
引用
收藏
页码:326 / 332
页数:7
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