Restricted cell elongation in Arabidopsis hypocotyls is associated with a reduced average pectin esterification level

被引:151
作者
Derbyshire, Paul
McCann, Maureen C.
Roberts, Keith [1 ]
机构
[1] John Innes Ctr, Dept Metab Biol, Norwich NR4 7UH, Norfolk, England
[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[3] John Innes Ctr, Dept Cell & Dev Biol, Norwich NR4 7UH, Norfolk, England
关键词
D O I
10.1186/1471-2229-7-31
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Cell elongation is mainly limited by the extensibility of the cell wall. Dicotyledonous primary ( growing) cell walls contain cellulose, xyloglucan, pectin and proteins, but little is known about how each polymer class contributes to the cell wall mechanical properties that control extensibility. Results: We present evidence that the degree of pectin methyl-esterification ( DE%) limits cell growth, and that a minimum level of about 60% DE is required for normal cell elongation in Arabidopsis hypocotyls. When the average DE% falls below this level, as in two gibberellic acid ( GA) mutants ga1-3 and gai, and plants expressing pectin methyl-esterase ( PME1) from Aspergillus aculeatus, then hypocotyl elongation is reduced. Conclusion: Low average levels of pectin DE% are associated with reduced cell elongation, implicating PMEs, the enzymes that regulate DE%, in the cell elongation process and in responses to GA. At high average DE% other components of the cell wall limit GA-induced growth.
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页数:12
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