Enzymatic activity of the ribosome-bound nascent polypeptide

被引:65
作者
Makeyev, EV [1 ]
Kolb, VA [1 ]
Spirin, AS [1 ]
机构
[1] RUSSIAN ACAD SCI,INST PROT RES,PUSHCHINO 142292,RUSSIA
关键词
nascent peptide; enzymatic activity; luciferase;
D O I
10.1016/0014-5793(95)01438-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Firefly luciferase was shown to be completely folded and thus enzymatically active immediately upon release from the ribosome [Kolb et al. (1994) EMBO J. 13, 3631-3637]. However, no luciferase activity was observed while full-length luciferase was attached to the ribosome as a peptidyl-tRNA, probably because the C-terminal portion of the enzyme is masked by the ribosome and/or ribosome-associated proteins, Here we have demonstrated that the ribosome-bound enzyme acquires the enzymatic activity when its C-terminus is extended by at least 26 additional amino acid residues, The results demonstrate that the acquisition of the final native conformation by a nascent protein does not need the release of the protein from the ribosome.
引用
收藏
页码:166 / 170
页数:5
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