Intracellular localization of VDAC proteins in plants

被引：48

作者：

Clausen, C

Ilkavets, I

Thomson, R

Philippar, K

Vojta, A

Möhlmann, T

Neuhaus, E

Fulgosi, H

Soll, J

机构：

[1] Univ Munich, Dept Biol 1, D-80638 Munich, Germany

[2] Univ Kaiserslautern, Fachbereich Biol, D-67663 Kaiserslautern, Germany

来源：

PLANTA | 2004年 / 220卷 / 01期

关键词：

envelope membrane; mitochondrion; Pisum; plastid; solute transport; voltage-dependent anion channel;

D O I：

10.1007/s00425-004-1325-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Voltage-dependent anion channels (VDACs) are porin-type beta-barrel diffusion pores. They are prominent in the outer membrane of mitochondria and facilitate metabolite exchange between the organelle and the cytosol. Here we studied the subcellular distribution of a plant VDAC-like protein between plastids and mitochondria in green and non-green tissue. Using in vitro studies of dual-import into mitochondria and chloroplasts as well as transient expression of fluorescence-labeled polypeptides, it could be clearly demonstrated that this VDAC isoform targets exclusively to mitochondria and not to plastids. Our results support the idea that plastids evolved a concept of solute exchange with the cytosol different from that of mitochondria.

引用

页码：30 / 37

页数：8

共 43 条

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