Macrophages exposed to Mycobacterium tuberculosis release chemokines able to recruit selected leucocyte subpopulations:: focus on γδ cells

被引:98
作者
Ferrero, E
Biswas, P
Vettoretto, K
Ferrarini, M
Uguccioni, M
Piali, L
Leone, BE
Moser, B
Rugarli, C
Pardi, R
机构
[1] San Raffaele Sci Inst, Lab Tumor Immunol, I-20132 Milan, Italy
[2] San Raffaele Sci Inst, Clin Immunol Lab, I-20132 Milan, Italy
[3] San Raffaele Sci Inst, Pathol Lab, I-20132 Milan, Italy
[4] Univ Vita & Salute, Milan, Italy
[5] San Raffaele Sci Inst, DIBIT, Milan, Italy
[6] Univ Bern, Theodor Kocher Inst, Bern, Switzerland
[7] Univ Childrens Hosp, Dept Res, Basel, Switzerland
关键词
D O I
10.1046/j.1365-2567.2003.01600.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Granuloma is a typical feature of tuberculosis. We evaluated the chemotaxis of selected human leucocyte subsets induced by macrophages incubated with Mycobacterium tuberculosis (MT)-derived products in vitro . The release of monocyte chemotactic protein 1 (MCP-1) and interleukin-8 (IL-8) correlated with the specific induction of strong chemotaxis towards monocytes and polymorphonuclear leucocytes (PMNs). gammadelta and T helper type 1 (Th1) alphabeta lymphocytes were chemoattracted, while T-resting, IL-2-activated and Th2 lymphocytes were unaffected. Activation with mycobacterium-derived, phosphate-containing components, modulated the chemokine receptor profile of gammadelta T lymphocytes as well as their pattern of cyto-chemokine production, disclosing a potential for their active participation in granuloma formation. In particular, CXCR3 and IP-10, which we found to be released by MT-pulsed alveolar macrophages, seem to represent the receptor-counter-receptor pair implicated in the chemotaxis of gammadelta lymphocytes. Immunohistochemical analysis and in situ hybridization revealed the in vivo presence of IL-8, MCP-1 and IL-10 in lymph node and lung tuberculous granulomas. Our results underscore the role of MT extracts in the induction of macrophage-derived chemokines responsible for the orchestrated recruitment of PMNs, monocytes, and Th1 and gammadelta T cells, as well as in the regulation of gammadelta function.
引用
收藏
页码:365 / 374
页数:10
相关论文
共 43 条
[1]   Chemokines and leukocyte traffic [J].
Baggiolini, M .
NATURE, 1998, 392 (6676) :565-568
[2]  
Biswas P, 2000, EUR J IMMUNOL, V30, P2172, DOI 10.1002/1521-4141(2000)30:8<2172::AID-IMMU2172>3.0.CO
[3]  
2-P
[4]   TUBERCULOSIS - COMMENTARY ON A REEMERGENT KILLER [J].
BLOOM, BR ;
MURRAY, CJL .
SCIENCE, 1992, 257 (5073) :1055-1064
[5]   HUMAN V-GAMMA-9-V-DELTA-2 CELLS ARE STIMULATED IN A CROSS-REACTIVE FASHION BY A VARIETY OF PHOSPHORYLATED METABOLITES [J].
BURK, MR ;
MORI, L ;
DELIBERO, G .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1995, 25 (07) :2052-2058
[6]  
Cipriani B, 2000, BLOOD, V95, P39
[7]   STIMULATION OF HUMAN GAMMA-DELTA T-CELLS BY NONPEPTIDIC MYCOBACTERIAL LIGANDS [J].
CONSTANT, P ;
DAVODEAU, F ;
PEYRAT, MA ;
POQUET, Y ;
PUZO, G ;
BONNEVILLE, M ;
FOURNIE, JJ .
SCIENCE, 1994, 264 (5156) :267-270
[8]  
D'Ambrosio D, 1998, J IMMUNOL, V161, P5111
[9]  
DANNENBERG AM, 1998, TUBERCULOSIS PATHOGE, P459
[10]  
Dieli F, 2000, EUR J IMMUNOL, V30, P1512, DOI 10.1002/(SICI)1521-4141(200005)30:5<1512::AID-IMMU1512>3.0.CO