Rapid characterisation of deep-sea actinomycetes for biotechnology screening programmes

被引:22
作者
Colquhoun, JA
Zulu, J
Goodfellow, M
Horikoshi, K
Ward, AC
Bull, AT [1 ]
机构
[1] Univ Kent, Res Sch Biosci, Canterbury CT2 7NJ, Kent, England
[2] Newcastle Univ, Dept Agr & Environm Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[3] Japan Marine Sci & Technol Ctr, DeepStar Grp, Yokosuka, Kanagawa 237, Japan
来源
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2000年 / 77卷 / 04期
基金
英国生物技术与生命科学研究理事会;
关键词
actinomycetes; deep-sea; dereplication; pyrolysis mass spectrometry; screening;
D O I
10.1023/A:1002627314735
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A continual need in natural product discovery is dereplication, that is the ability to exclude previously tested microorganisms from screening programmes. Whole-cell fingerprinting techniques offer an ideal solution to this problem because of their rapidity and reproducibility, dependence on small samples, and automation. One such technique, Curie-point pyrolysis mass spectrometry (PyMS), has been deployed for the characterisation of a unique collection of actinomycetes recovered from Pacific Ocean sediments approximately 2000 to 6500 m below sea level. This paper addresses the question: to what extent are pyrogroups, defined on the basis of PyMS fingerprinting, related to classifications derived from more conventional microbial systematics? A collection of 44 randomly chosen deep-sea rhodococci were coded and subjected to a double-blind PyMS and numerical taxonomic (NT) analysis; the latter sorted the strains into clusters (taxospecies) using large sets of equally weighted phenotypic data. At the end of the experiment the codes were disclosed and the NT classification shown to generate 6 homogeneous clusters corresponding to different deep-sea sites. The matching of these clusters with the resulting pyrogroups was very high with an overall congruence of nearly 98%. Thus, PyMS characterisation is directly ascribable to the phenotypic variation being sought for biotechnology screens. Moreover, the exquisite discriminatory power of PyMS readily revealed infraspecific diversity in these industrially important bacteria.
引用
收藏
页码:359 / 367
页数:9
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