Secretory Profiles and Wound Healing Effects of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

被引：154

作者：

Yoon, Byung Sun ^{[1
,2
]}

Moon, Jai-Hee ^{[1
]}

Jun, Eun Kyoung ^{[1
,4
]}

Kim, Jonggun ^{[3
]}

Maeng, Isaac ^{[1
]}

Kim, Jun Sung ^{[1
]}

Lee, Jung Han ^{[1
,4
]}

Baik, Cheong Soon ^{[5
]}

Kim, Aeree ^{[6
]}

Cho, Kyoung Shik ^{[4
]}

Lee, Jang Ho ^{[4
]}

Lee, Hwang Heui ^{[4
,7
]}

Whang, Kwang Youn ^{[3
]}

You, Seungkwon ^{[1
]}

机构：

[1] Korea Univ, Lab Cell Funct Regulat, Coll Life Sci & Biotechnol, Seoul 136701, South Korea

[2] Korea Univ, Inst Life Sci & Nat Resources, Seoul 136701, South Korea

[3] Korea Univ, Div Biotechnol, Coll Life Sci & Biotechnol, Seoul 136701, South Korea

[4] Stemmedience Corp, Div Stem Cell, Res Inst, Seoul, South Korea

[5] SamKwang Med Labs, Seoul, South Korea

[6] Korea Univ, Guro Hosp, Dept Pathol, Coll Med, Seoul 136701, South Korea

[7] ChungDam NB Clin, Seoul, South Korea

来源：

STEM CELLS AND DEVELOPMENT | 2010年 / 19卷 / 06期

关键词：

UMBILICAL-CORD BLOOD; HEPATOCYTE GROWTH-FACTOR; BONE-MARROW; STROMAL CELLS; IN-VITRO; EXPRESSION; DIFFERENTIATION; ADULT; CYTOKINES; GENES;

D O I：

10.1089/scd.2009.0138

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to accelerate the wound-healing process by stimulating proliferation and migration of dermal fibroblasts. AF-MSCs expressed the typical MSC marker proteins CD13, CD29, and CD44 and differentiated into adipocytes, osteoblasts, and chondrocytes when exposed to the appropriate differentiation media. In addition, AF-MSC-conditioned media (AF-MSC-CM) significantly enhanced proliferation of dermal fibroblasts. Antibody-based protein array and enzyme-linked immunosorbent assay (ELISA) indicated that AF-MSC-CM contains various cytokines and chemokines that are known to be important in normal wound healing, including IL-8, IL-6, TGF-beta, TNFRI, VEGF, and EGF. Application of AF-MSC-CM significantly enhanced wound healing by dermal fibroblasts via the TGF-beta/SMAD2 pathway. Levels of p-SMAD2 were increased by AF-MSC-CM, and both the increase in p-SMAD2 and migration of dermal fibroblasts were blocked by inhibiting the TGF-beta/SMAD2 pathway. Moreover, in a mouse excisional wound model, AF-MSC-CM accelerated wound healing. These data provide the first evidence of the potential for AF-MSC-CM in the treatment of skin wounds.

引用

页码：887 / 902

页数：16

共 69 条

[11] Paracrine Factors of Mesenchymal Stem Cells Recruit Macrophages and Endothelial Lineage Cells and Enhance Wound Healing
Chen, Liwen
Tredget, Edward E.
Wu, Philip Y. G.
Wu, Yaojiong
[J]. PLOS ONE, 2008, 3 (04):
[12] Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells
Colter, DC
Sekiya, I
Prockop, DJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (14) : 7841 - 7845
[13] Isolation of amniotic stem cell lines with potential for therapy
De Coppi, Paolo
Bartsch, Georg, Jr.
Siddiqui, M. Minhaj
Xu, Tao
Santos, Cesar C.
Perin, Laura
Mostoslavsky, Gustavo
Serre, Angeline C.
Snyder, Evan Y.
Yoo, James J.
Furth, Mark E.
Soker, Shay
Atala, Anthony
[J]. NATURE BIOTECHNOLOGY, 2007, 25 (01) : 100 - 106
[14] Nonviral transfer of genes to pig primary keratinocytes. Induction of angiogenesis by composite grafts of modified keratinocytes overexpressing VEGF driven by a keratin promoter
Del Rio, M
Larcher, F
Meana, A
Segovia, JC
Alvarez, A
Jorcano, JL
[J]. GENE THERAPY, 1999, 6 (10) : 1734 - 1741
[15] ABC of wound healing - Non-surgical and drug treatments
Enoch, S
Grey, JE
Harding, KG
[J]. BRITISH MEDICAL JOURNAL, 2006, 332 (7546): : 900 - 903
[16] Amniotic fluid and placental stem cells
Fauza, D
[J]. BEST PRACTICE & RESEARCH CLINICAL OBSTETRICS & GYNAECOLOGY, 2004, 18 (06) : 877 - 891
[17] The CXC chemokine cCAF stimulates differentiation of fibroblasts into myofibroblasts and accelerates wound closure
Feugate, JE
Li, QJ
Wong, L
Martins-Green, M
[J]. JOURNAL OF CELL BIOLOGY, 2002, 156 (01) : 161 - 172
[18] Cytokine expression profile over time in severely burned pediatric patients
Finnerty, Celeste C.
Herndon, David N.
Przkora, Rene
Pereira, Clifford T.
Oliveira, Hermes M.
Queiroz, Dulciene M. M.
Rocha, Andreia M. C.
Jeschke, Marc G.
[J]. SHOCK, 2006, 26 (01): : 13 - 19
[19] c-Abl is an effector of Src for growth factor-induced c-myc expression and DNA synthesis
Furstoss, O
Dorey, K
Simon, V
Barilà, D
Superti-Furga, G
Roche, S
[J]. EMBO JOURNAL, 2002, 21 (04) : 514 - 524
[20] Quantitative and reproducible murine model of excisional wound healing
Galiano, RD
Michaels, J
Dobryansky, M
Levine, JP
Gurtner, GC
[J]. WOUND REPAIR AND REGENERATION, 2004, 12 (04) : 485 - 492

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