Antibodies and T cells against synthetic peptides of the C-terminal domain (Hc) of botulinum neurotoxin type A and their cross-reaction with Hc

被引:28
作者
Oshima, M
Middlebrook, JL
Atassi, MZ
机构
[1] Baylor Coll Med, Dept Biochem, Houston, TX 77030 USA
[2] USA, Div Life Sci, Dugway, UT 84022 USA
关键词
botulinum neurotoxin; synthetic peptides; antibodies; T cells; epitopes;
D O I
10.1016/S0165-2478(97)00124-7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Seventeen peptides containing T cell and/or antibody (Ab) epitopes previously localized on H-c of botulinum neurotoxin type A were used in SJL and BALB/c mice as immunogens either individually or as an equimolar mixture of groups that contained epitopes of T cells, Abs or both, to determine their abilities to generate T cells and/or Abs that recognize intact H-c. In SJL, peptide 897-915 which included both T cell and Ab epitopes, elicited Abs that cross-reacted very strongly with H-c. In BALB/c, peptides 869-887, 883-901, 981-999 and 1275-1296 which contained Ab epitopes generated Abs that cross-reacted strongly with H-c. A mixture of peptides that contained T cell and Ab epitopes was effective in both strains in eliciting T cells and Abs that cross-reacted with H-c. This mixture form gave a quicker rise (after two injections) in cross-reactive (with H-c) Ab titer as compared to other peptide mixtures or the individual peptides, and sustained in BALB/c a high Ab titer upon further booster injections. Some of the regions that elicited crossreactive immunity to H-c have sequence similarity to other clostridial toxins, suggesting that one or more of these synthetic peptides might provide cross-protection against those toxins. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:7 / 12
页数:6
相关论文
共 21 条
[21]   INACTIVATION OF CLOSTRIDIUM-BOTULINUM TYPE-A NEUROTOXIN BY TRYPSIN AND PURIFICATION OF 2 TRYPTIC FRAGMENTS - PROTEOLYTIC ACTION NEAR THE COOH-TERMINUS OF THE HEAVY SUBUNIT DESTROYS TOXIN-BINDING ACTIVITY [J].
SHONE, CC ;
HAMBLETON, P ;
MELLING, J .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 151 (01) :75-82