A hierarchical self-assembly of eumelanin

被引:76
作者
Clancy, CMR
Nofsinger, JB
Hanks, RK
Simon, JD [1 ]
机构
[1] Duke Univ, Dept Chem, Durham, NC 27708 USA
[2] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA
关键词
D O I
10.1021/jp001630q
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Atomic force microscopy is used to examine the structure of a natural eumelanin isolated from the ink sacs of cuttlefish (Sepia officinalis). The experimental data presented clearly show that the 100-200 nm spherical eumelanin particles imaged previously by SEM are not a fundamental structural unit. While these spherical particles are stable structures, as is evidenced by their cohesiveness under mechanical stress, the AFM images reveal that these particles are composed of smaller constituents. Taking recent scattering and mass spectrometry results into consideration, we conclude that the self-assembly of Sepia eumelanin is a hierarchical process with small units assembling into hundred-nanometer structures, which then aggregate to form the morphology of the macroscopic pigment.
引用
收藏
页码:7871 / 7873
页数:3
相关论文
共 19 条
[11]  
2-Y
[12]   Probing the building blocks of eumelanins using scanning electron microscopy [J].
Nofsinger, JB ;
Forest, SE ;
Eibest, LM ;
Gold, KA ;
Simon, JD .
PIGMENT CELL RESEARCH, 2000, 13 (03) :179-184
[13]  
Pezzella A, 1997, RAPID COMMUN MASS SP, V11, P368, DOI 10.1002/(SICI)1097-0231(19970228)11:4<368::AID-RCM859>3.0.CO
[14]  
2-E
[15]  
Sealy R.C., 1980, FREE RADICAL BIO MED, V4, P209, DOI DOI 10.1016/B978-0-12-566504-9.50014-3
[16]   PHEOMELANIN AS WELL AS EUMELANIN IS PRESENT IN HUMAN EPIDERMIS [J].
THODY, AJ ;
HIGGINS, EM ;
WAKAMATSU, K ;
ITO, S ;
BURCHILL, SA ;
MARKS, JM .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (02) :340-344
[17]   THE FUNDAMENTAL UNIT OF SYNTHETIC MELANIN - A VERIFICATION BY TUNNELING MICROSCOPY OF X-RAY-SCATTERING RESULTS [J].
ZAJAC, GW ;
GALLAS, JM ;
CHENG, J ;
EISNER, M ;
MOSS, SC ;
ALVARADOSWAISGOOD, AE .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1994, 1199 (03) :271-278
[18]   TUNNELING MICROSCOPY VERIFICATION OF AN X-RAY SCATTERING-DERIVED MOLECULAR-MODEL OF TYROSINE-BASED MELANIN [J].
ZAJAC, GW ;
GALLAS, JM ;
ALVARADOSWAISGOOD, AE .
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1994, 12 (03) :1512-1516
[19]   MELANIN STANDARD METHOD - PARTICLE DESCRIPTION [J].
ZEISE, L ;
MURR, BL ;
CHEDEKEL, MR .
PIGMENT CELL RESEARCH, 1992, 5 (03) :132-142