Identification of lorazepam and sildenafil as examples for the application of LC/ionspray-MS and MS-MS with mass spectra library searching in forensic toxicology

被引：29

作者：

Weinmann, W

Lehmann, N

Müller, C

Wiedemann, A

Svoboda, M

机构：

[1] Univ Freiburg Klinikum, Inst Forens Med, D-79104 Freiburg, Germany

[2] PE Biosyst, D-63225 Langen, Germany

来源：

FORENSIC SCIENCE INTERNATIONAL | 2000年 / 113卷 / 1-3期

关键词：

LC-MS; tandem mass spectrometry; mass spectra libraries; drugs; lorazepam; sildenafil;

D O I：

10.1016/S0379-0738(00)00256-5

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A mass spectra (MS) library using in-source collision induced dissociation (ESI-CID) as well as a tandem-mass spectra (MS-MS) library with product ion spectra of drugs has recently been developed with a triple-quadrupole ionspray mass spectrometer [1,2]. For the ESI-CID MS library, single-quadrupole mode and for the MS-MS library triple-quadrupole mode have been used. These mass spectra libraries were applied successfully for the general-unknown screening for drugs and metabolites in serum and urine with liquid-chromatography-mass spectrometry (LC-MS) using a PE/SCIEX API 365 with a turboionspray source. As examples, the identification of lorazepam and lorazepam-glucuronide in a serum extract and the identification of sildenafil and alkyloxidated sildenafil in urine are presented here. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：339 / 344

页数：6

共 12 条

[1] Poor reproducibility of in-source collisional atmospheric pressure ionization mass spectra of toxicologically relevant drugs [J].