Protein crystallization and phase diagrams

被引：214

作者：

Asherie, N

机构：

[1] MIT, Dept Phys, Ctr Mat Sci & Engn, Cambridge, MA 02139 USA

[2] MIT, Dept Phys, Ctr Mat Proc, Cambridge, MA 02139 USA

来源：

METHODS | 2004年 / 34卷 / 03期

关键词：

protein crystals; liquid-liquid phase separation; phase diagrams; cloud-point method; solubility;

D O I：

10.1016/j.ymeth.2004.03.028

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The phase diagram is a map which represents the state of a material (e.g., solid and liquid) as a function of the ambient conditions (e.g., temperature and concentration). It is therefore a useful tool in processing many different classes of materials. In this article, methods to determine the phase diagram of an aqueous solution of a globular protein are described, focusing on the solid (crystal) and condensed liquid states. The use of the information contained in the phase diagram for protein crystallization is also discussed. (C) 2004 Elsevier Inc. All rights reserved.

引用

收藏

页码：266 / 272

页数：7

相关论文

共 57 条

[1]

ALBER T, 1981, J BIOL CHEM, V256, P1356

[2] Observation of liquid-liquid phase separation for eye lens γS-crystallin [J].

Annunziata, O ;

Ogun, O ;

Benedek, GB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (03) :970-974

[3] Enhanced crystallization of the Cys18 to Ser mutant of bovine γB crystallin [J].

Asherie, N ;

Pande, J ;

Pande, A ;

Zarutskie, JA ;

Lomakin, J ;

Lomakin, A ;

Ogun, O ;

Stern, LJ ;

King, J ;

Benedek, GB .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 314 (04) :663-669

[4] Phase diagram of colloidal solutions [J].

Asherie, N ;

Lomakin, A ;

Benedek, GB .

PHYSICAL REVIEW LETTERS, 1996, 77 (23) :4832-4835

[5] SYSTEMATIC STUDIES ON THE CRYSTALLIZATION OF LYSOZYME - DETERMINATION AND USE OF PHASE-DIAGRAMS [J].

ATAKA, M ;

ASAI, M .

JOURNAL OF CRYSTAL GROWTH, 1988, 90 (1-3) :86-93

[6]

Benedek GB, 1997, INVEST OPHTH VIS SCI, V38, P1911

[7] SOLID LIQUID-PHASE BOUNDARIES OF LENS PROTEIN SOLUTIONS [J].

BERLAND, CR ;

THURSTON, GM ;

KONDO, M ;

BROIDE, ML ;

PANDE, J ;

OGUN, O ;

BENEDEK, GB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (04) :1214-1218

[8] SOLUBILITY, PHASE-TRANSITION, KINETIC RIPENING AND GROWTH-RATES OF PORCINE PANCREATIC ALPHA-AMYLASE ISOENZYMES [J].

BOISTELLE, R ;

ASTIER, JP ;

MARCHISMOUREN, G ;

DESSEAUX, V ;

HASER, R .

JOURNAL OF CRYSTAL GROWTH, 1992, 123 (1-2) :109-120

[9] Hybrid insulin cocrystals for controlled release delivery [J].

Brader, ML ;

Sukumar, M ;

Pekar, AH ;

McClellan, DS ;

Chance, RE ;

Flora, DB ;

Cox, AL ;

Irwin, L ;

Myers, SR .

NATURE BIOTECHNOLOGY, 2002, 20 (08) :800-804

[10] BINARY-LIQUID PHASE-SEPARATION OF LENS PROTEIN SOLUTIONS [J].

BROIDE, ML ;

BERLAND, CR ;

PANDE, J ;

OGUN, OO ;

BENEDEK, GB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (13) :5660-5664

← 1 2 3 4 5 6 →