Rapid detection directly from patient serum samples of human cytomegalovirus UL97 mutations conferring ganciclovir resistance

被引：34

作者：

Castor, Jared

Cook, Linda

Corey, Lawrence

Jerome, Keith R.

机构：

[1] Fred Hutchinson Canc Res Ctr, Program Infect Dis, Seattle, WA 98109 USA

[2] Univ Washington, Dept Lab Med, Seattle, WA 98195 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 08期

关键词：

D O I：

10.1128/JCM.00526-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ganciclovir-resistant cytomegalovirus can cause disease and death in transplant recipients. We describe here a rapid PCR- and sequencing-based assay for ganciclovir resistance that can be performed in I to 2 working days directly from patient specimens, without the need for amplification of the virus by cell culture. An evaluation of 120 sequential samples submitted for clinical testing revealed a variety of silent and amino acid mutations.

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页码：2681 / 2683

页数：3

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