ISOLATION OF MICROSATELLITE MARKERS FOR THE COMMON MEDITERRANEAN SHRUB MYRTUS COMMUNIS (MYRTACEAE)

被引:14
作者
Albaladejo, Rafael G. [1 ]
Sebastiani, Federico [2 ]
Gonzalez-Martinez, Santiago C. [3 ]
Gonzalez-Varo, Juan P. [1 ]
Vendramin, Giovanni G. [4 ]
Aparicio, Abelardo [1 ]
机构
[1] Univ Seville, Dept Biol Vegetal & Ecol, E-41012 Seville, Spain
[2] Univ Florence, Dipartimento Biotecnol Agr, I-50019 Florence, Italy
[3] CIFOR INIA, Dept Forest Ecol & Genet, Madrid 28040, Spain
[4] CNR, Ist Genet Vegetale, I-50019 Florence, Italy
关键词
gene flow; habitat fragmentation; Mediterranean landscapes; microsatellites; Myrtus communis;
D O I
10.3732/ajb.1000060
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Premise of the study : The development of microsatellite markers was conducted in the Mediterranean common shrub Myrtus communis (myrtle) to assess levels of genetic diversity and patterns of gene flow across fragmented landscapes in southern Spain. Methods and Results : Fourteen primer pairs were isolated showing clear and consistent patterns of amplification, three of which were apparently monomorphic. Levels of polymorphism in the other 11 markers were checked in 48 individuals from two populations. The number of alleles per locus ranged from 3 to 11 and the total number of alleles was 83. Conclusions : These highly polymorphic markers will allow us to improve our understanding of the genetic consequences of chronic fragmentation in Mediterranean landscapes.
引用
收藏
页码:E23 / E25
页数:3
相关论文
共 10 条
[1]   Population genetic structure in Myrtus communis L. in a chronically fragmented landscape in the Mediterranean: can gene flow counteract habitat perturbation? [J].
Albaladejo, R. G. ;
Carrillo, L. F. ;
Aparicio, A. ;
Fernandez-Manjarres, J. F. ;
Gonzalez-Varo, J. P. .
PLANT BIOLOGY, 2009, 11 (03) :442-453
[2]   MAXIMUM LIKELIHOOD FROM INCOMPLETE DATA VIA EM ALGORITHM [J].
DEMPSTER, AP ;
LAIRD, NM ;
RUBIN, DB .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-METHODOLOGICAL, 1977, 39 (01) :1-38
[3]   Valuing Common Species [J].
Gaston, Kevin J. .
SCIENCE, 2010, 327 (5962) :154-155
[4]   Mating patterns and spatial distribution of conspecific neighbours in the Mediterranean shrub Myrtus communis (Myrtaceae) [J].
Gonzalez-Varo, Juan P. ;
Albaladejo, Rafael G. ;
Aparicio, Abelardo .
PLANT ECOLOGY, 2009, 203 (02) :207-215
[5]   Susceptibility of common and rare plant species to the genetic consequences of habitat fragmentation [J].
Honnay, Olivier ;
Jacquemyn, Hans .
CONSERVATION BIOLOGY, 2007, 21 (03) :823-831
[6]   GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research [J].
Peakall, R ;
Smouse, PE .
MOLECULAR ECOLOGY NOTES, 2006, 6 (01) :288-295
[7]   GENEPOP′007:: a complete re-implementation of the GENEPOP software for Windows and Linux [J].
Rousset, Francois .
MOLECULAR ECOLOGY RESOURCES, 2008, 8 (01) :103-106
[8]  
Rozen S, 2000, Methods Mol Biol, V132, P365
[9]   An economic method for the fluorescent labeling of PCR fragments [J].
Schuelke, M .
NATURE BIOTECHNOLOGY, 2000, 18 (02) :233-234
[10]   Transferability of microsatellite markers from Eucalyptus, spp. to Eugenia dysenterica (Myrtaceae family) [J].
Zucchi, MI ;
Brondani, RPV ;
Pinheiro, JB ;
Brondani, C ;
Vencovsky, R .
MOLECULAR ECOLOGY NOTES, 2002, 2 (04) :512-513